Question

Above are bacteria expressing the green fluorescent protein (GFP) originally cloned from jelly fish. The GFP gene has been inserted into the lac operon such that when the lac operon is ON, the GFP protein will be expressed. The cells above are from a colony. 1. What does the sentence above mean by "inserted into the lac operon"? Draw a figure that explains that sentence mote S 个ャ epressov inding Polymerus CAP 2. A colony of bacteria are grown from a single cell. Therefore, are all the cells above identi- cal? Explain.

I need #2, 3, and 4 please!

Answer 1

2. Yes, they are identical because they are formed from the single cell or clone of cells of similar genetic makeup and also, we take the pure culture of that bacteria for expression.

3. A: In the media, it should have lactose or allolactose or IPTG

   B: Glucose must be absent in the media

4.

For expression of GFP protein in cells need to have inducer in the form of lactose or allolactose or IPTG. For transport of inducer into the cells requires permease transporter protein, may be the unexpressed cell don’t have permease enzyme.

Other reasons

Some of the cells are not expressed the GFP linked protein in the cells even they are clone of same cells

The insertion of GFP protein into your desired gene not done completely or not inserted, So the non-inserted GFP protein cells could not give green fluorescence.