Homework Answers
Disadvantages of using non-homologous joining mechanism to repair a double strand break damage are - as it requires homologous sequence to guide repair, overhangs are perfectly incompatible, inappropriate NHEJ can leads to translocations and telomere fusions, in case of bacteria template for recombination is not available, yeast lacking pol4 are unable to join 3' overhangs which requires gap filling, low levels of end joining can still occur.
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Question 7 2 pts What is or are the disadvantages of using nonhomologous joining mechanism to...
Question 5 1 pts Non-homologous end joining is "error prone" because There is no template used...
Question 5 1 pts Non-homologous end joining is "error prone" because There is no template used during repair The DNA polymerase invovled adds incorrect bases ODNA ends are often trimmed before repair Damaged bases are incorrectly paired during repair Question 6 1 pts Global genomic and transcription coupled are the two sub-types of nucleotide excision repair. Select true statements relevant to these processes Both can detect damage involving multiple damaged bases Both involve proteins that recognize the damaged bases Both...
please help answer these questions 3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based...
please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
Question 4 4 pts Which statement best describes mismatch repair mechanism uracile glcosylase is part of...
Question 4 4 pts Which statement best describes mismatch repair mechanism uracile glcosylase is part of this mechanism. An light-dependent enzyme is involved. can couple with homologous recombination to repair DS break Deamination is the cause of this DNA damage.
Question 7 2 pts Which of these genes would not likely be regulated by the bacterial...
Question 7 2 pts Which of these genes would not likely be regulated by the bacterial SOS response?! Translesion DNA polymerase Cell division promoter Holliday junction branch migration enzyme Nucleotide excision repair enzyme Question 8 2 pts Which of these mutations is likely to have the greatest impact on the amino acid composition of the resulting protein? Silent Synonymous Frameshift Missense Question 9 2 pts What would be the result of perfect and continuous suppression of the lac operon in...
17. A diploid plant is a dihybrid for flower color (gene “R”) and leaf size (gene...
17. A diploid plant is a dihybrid for flower color (gene “R”)
and leaf size (gene “L”). Its
phenotype is red flowers and large leaves. This plant is crossed to
a tester plant (which has
white flowers and small leaves). The progeny is as follows:
23 Red, large
25 White, small
230 Red, small
235 White, large
What can be concluded about the linkage relationship of the loci in
the dihybrid parent?
A) Genes R and L are unlinked
B)...
q39 Question 39 0.35 pts Homologous recombination is an important mechanism in which organisms use a...
q39
Question 39 0.35 pts Homologous recombination is an important mechanism in which organisms use a "backup" copy of the DNA as a template to fix double-strand breaks without loss of genetic information. Which of the following is necessary for homologous recombination to occur? telomerase O 3 DNA strand overhangs • a short stretch of sequence similarity no DNA strand overhangs
Question 1 4 pts Which characteristic listed below is not shared between homologous recombination in Holiday...
Question 1 4 pts Which characteristic listed below is not shared between homologous recombination in Holiday model and doubled-strand (DS) DNA repair mechanism. ODS break locates on the same chromosome. Branch migration Strand invasion or exchange. Presence of sister chromatids.
4. The CRISPR-Cas9 system is an important new technique in molecular biology. What is the natural...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
Question 16 0.5 pts Identify the correct sequence of events proposed by the double-stranded break model...
Question 16 0.5 pts Identify the correct sequence of events proposed by the double-stranded break model of homologous recombination repair mechanisms: 1. Strand invasion and D loop formation. II. DNA gap repair synthesis. III. Generation of single stranded DNA segments. IV. Resolution. I, III, II, IV O III, I, II, IV III, II, I, IV IV, III, 1, 11 None of the above
can someone help me Question 7 1 pts Acute inflammation is a defense mechanism of the...
can someone help me
Question 7 1 pts Acute inflammation is a defense mechanism of the immune system. All of the following is a benefit of inflammation except ONE. Select the exception. enlargement of blood vessels inflammation triggers antimicrobial peptides O increases the rate of tisssue repair diapedesis
Question 5 1 pts Non-homologous end joining is "error prone" because There is no template used during repair The DNA polymerase invovled adds incorrect bases ODNA ends are often trimmed before repair Damaged bases are incorrectly paired during repair Question 6 1 pts Global genomic and transcription coupled are the two sub-types of nucleotide excision repair. Select true statements relevant to these processes Both can detect damage involving multiple damaged bases Both involve proteins that recognize the damaged bases Both...
please help answer these questions
3. What are the similarities, differences, advantages, and disadvantages of CRISPR-based gene editing versus Zinc-finger nucleases and TALENS? 4. What is crRNA and what does it do? 5. What is tracrRNA and what does it do? 6. What is the PAM sequence and what is its significance? 7. What can nuclease-deficient Cas9 (acas) proteins be used for? 8. You want to insert DNA encoding an epitope tag to the end of a specific gene you...
Question 4 4 pts Which statement best describes mismatch repair mechanism uracile glcosylase is part of this mechanism. An light-dependent enzyme is involved. can couple with homologous recombination to repair DS break Deamination is the cause of this DNA damage.
Question 7 2 pts Which of these genes would not likely be regulated by the bacterial SOS response?! Translesion DNA polymerase Cell division promoter Holliday junction branch migration enzyme Nucleotide excision repair enzyme Question 8 2 pts Which of these mutations is likely to have the greatest impact on the amino acid composition of the resulting protein? Silent Synonymous Frameshift Missense Question 9 2 pts What would be the result of perfect and continuous suppression of the lac operon in...
17. A diploid plant is a dihybrid for flower color (gene “R”)
and leaf size (gene “L”). Its
phenotype is red flowers and large leaves. This plant is crossed to
a tester plant (which has
white flowers and small leaves). The progeny is as follows:
23 Red, large
25 White, small
230 Red, small
235 White, large
What can be concluded about the linkage relationship of the loci in
the dihybrid parent?
A) Genes R and L are unlinked
B)...
q39
Question 39 0.35 pts Homologous recombination is an important mechanism in which organisms use a "backup" copy of the DNA as a template to fix double-strand breaks without loss of genetic information. Which of the following is necessary for homologous recombination to occur? telomerase O 3 DNA strand overhangs • a short stretch of sequence similarity no DNA strand overhangs
Question 1 4 pts Which characteristic listed below is not shared between homologous recombination in Holiday model and doubled-strand (DS) DNA repair mechanism. ODS break locates on the same chromosome. Branch migration Strand invasion or exchange. Presence of sister chromatids.
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
Question 16 0.5 pts Identify the correct sequence of events proposed by the double-stranded break model of homologous recombination repair mechanisms: 1. Strand invasion and D loop formation. II. DNA gap repair synthesis. III. Generation of single stranded DNA segments. IV. Resolution. I, III, II, IV O III, I, II, IV III, II, I, IV IV, III, 1, 11 None of the above
can someone help me
Question 7 1 pts Acute inflammation is a defense mechanism of the immune system. All of the following is a benefit of inflammation except ONE. Select the exception. enlargement of blood vessels inflammation triggers antimicrobial peptides O increases the rate of tisssue repair diapedesis
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