Question

The mRNA sequence below is the full length of the mature mRNA transcript. On the sequence, the 5'cap is indicated by (5'). The poly (A) tail is not shown. This transcript arrives at a ribosome. Determine the anticodon sequence for the first three tRNA used to make the polypeptide. Label the 5' and 3’ends on the tRNAs. 5' AAUCGAUGUAAUCCGCAUC 3

Answer 1

The anticodon sequence for the first three tRNA used to make the polypeptide are

5' - AAU CGA UGU AAU CCG CAU C - 3' - Given sequence

The first codon in the given sequence is 5' - AAU - 3'

The first anticodon sequence for the first tRNA is 3' - UUA - 5'

The second codon in the given sequence is 5' - CGA - 3'

The second anticodon sequence for the second tRNA is 3' - GCU - 5'

The third codon in the given sequence is 5' - UGU - 3'

The third anticodon sequence for the third tRNA is 3' - ACA - 5'

So, the given sequence runs from 5' - 3' and the tRNA runs complementary to the mRNA transcript and that is 3' - 5'

The anticodon sequence for the first three tRNA used to make the polypeptide is 3' - UUA GCU ACA ......... - 5'