Question

of none of the above QUESTION 10 The Uribe and Jagendorf acid bath experiment provided the first direct evidence in support of Mitchell's chemiosmotic coupling hypothesis. Which of the following is correct regarding the experiment? O It was conducted using chloroplasts membranes o It showed that a pH gradient could drive ATP synthesis separate from electron transport o It was camed out using mitochondna membranes o It was conducted using intact bacteria O Both A and B above of Both C and D above. QUESTION 1 nur any one and talim 1 un arcons

Answer 1

Option E is the correct answer...

Bothe A and B above is correct answer...because

Option A   It was done using chloroplast membrane

OptionB,    It showed that pH gradient could drive ATP synthesis seprate from ATP .

In the uribe and jagendort experiment created to the pH gradient outside of the membrane of thylocoid to incubated in the  chloroplasts pH 4 buffer for 15 sec. then chloroplasts was placed in the pH 8 buffer that contains  ADP and Pi. so the pH of the stroma increased to the 8, but the thylakoid lumen pH remains at 4. the pH difference it consists as  immediate increased in ATP synthesis followed by the proton gradient.