Question

Of the following choices, the best way to determine the activity of an enzyme is: A. Calculate the Vmax and of the Vmax activity OB. Plot substrate concentration on the x-axis and amount of product formed on the y-axis. The slope-rate. OC. Determine the activation energy (Ea) without the enzyme and then measure the Ea with the enzyme added. The difference in free energy is the activity D. Calculate amount of product formed at different times. Plot the amount of product formed on the yaads at each of the chosen times on the x-axis. The initial slope is the activity

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Answer 1

1.The activity of enzyme is a measure of the quantity of active enzyme present in any reaction, and it can be determined by the moles of substrate converted per unit time and reaction volume and by using the activation energy Ea, so the best way to determine the activity of enzyme is to calculate or find out the activation energy without enzyme and then calculate the activation energy using the enzyme,...    2. Proton motive force;;-proton motive force is the that force which promotes the movement of protons across the membrane, and yes it is similar to the H+ influx because there is the generation of ATP during the pmf so H+ influx play a very important role and it may be similar to the influx ., . 3. From the given options only option d is correct that is ATP is made in the lumen of chloroplast , that is all the electron transport process occur in the thylakoid membrane to make ATP ,H+ is pumped into thylakoid membrane space and backflow of the H+ through an ATP synthase produces the ATP in the chloroplast,.. 4.The plasma membrane is the cellular membrane which consists of both lipids and proteins,and the fundamental structure is the phospholipid bilayer which form the stable barrier ,so being barrier and presence of protein the bilayer has charged headgroups., so option a is correct. 5.Cilia are a minute hairlike organelles which helps in the movement and cilia are mainly desinged either to move the cell itself or over the any substance, the main purpose of cilia in mammalian cells is to move fluid, mucous or cells over their surface and its due to the centrilocular protein . 6.The anaerobic fermentation is a method by which cells use to extract energy from carbohydrates when oxygen is not present,   there are the formation of yeast in the muscle cells,. 7.The electron and proton are called as the secret of life  because both are helpfull in the creation of energy and option d is correct.