Question

Below are some of the steps that occur in a Nextgen genomic sequencing reaction. Fill in the missing steps. Steps MUST be in the proper order. a. Isolate and fragment genomic DNA from an organism Ev [ Select ] Add primer/ fluorescent chain terminating nucleotides Bind to chip Assemble contig Remove chain terminator Read fluorescence Ligate linkers C e. (Select ] f. Wash away excess, non-bound chain terminating nucleotides [Select] h. (Select ] Replication cycle is repeated until sequencing is complete. j. [Select]

(blocked) d. PCR amplification of DNA fragments, cluster growth

Answer 1

The missing steps are as follows:

b. Ligate linkers

c. Bind to chip

e. Add primer / fluorescent chain terminating nucleotides.

g. Read fluorescence

h. Remove chain terminator

j. Assemble contig