Sequences from Picture:
1. A C T T C T T G C C A C T G G T T G C T G C A A
2. G A G T A A T A C A G A G C G A G A G G A C G A C A
3. A T T G C T C C A G A T T G C A A
4. T C T A C T T C T T C T T C A C A T C T A C T A T A T C TA T C A C A G C G T A
MY QUESTION:
5. Are the sequences above identical to the DNA Template? Why or why not? -1pt
Homework Answers
Answer-5 no the sequences above are not identical to the template DNA sequence because these all are different from one another they doesn't share the similar nucleotide sequence.
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided,...
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its KEY corresponding peak. - 2pts And memes 1. Wallet 2 banan sam hesalesanan Amalan 3. 4. 12 V Α' Α' Aav po e te AL А. ab x *² ADA Style 5. Are the sequences above identical to the DNA Template? Why or why not? -1pt
Using the key provided (in the first photo on the right hand side) interpret the nucleotide...
Using the key provided (in the first photo on the right hand
side) interpret the nucleotide base sequences in the gour
electropherograms below. Write each base below its corresponding
peak.
Also, are the sequences above idential to the DNA template? Why or
why not?
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its corresponding peak. - 2pts KEY...
Directions: Using the key provided, interpret the nucleotide base ssion 1) sequences in the four electropherograms...
Directions: Using the key provided, interpret the nucleotide base ssion 1) sequences in the four electropherograms below. Write each base below its corresponding peaked interpret the the 2pts mucodebase sunces in the four electroplerograms below. Write in each base below its corresponding peak KEY banana Selebobolanie brake 5. Are the sequences above identical to the DNA Template? Why or why not? -1pt 6. What are the five main components of a PCR-based dye terminator reaction? -2.5pts
Tricky Question: For a phylogeny if you compare two sequences from two different species of zebras:...
Tricky Question: For a phylogeny if you compare two sequences from two different species of zebras: Zebra 1: A C T T G C A T C Zebra 2: A C T C G C A T C the assumption is that the traits at each location in a sequence, represented by the nucleotide letter (A, T, C, G), is... __________________ to the matched DNA nucleotide in the other sequence or other sequences. In other words, that DNA base has...
Problem 1. DNA sequences consist of four letters (also called bases): A, T, C, and G....
Problem 1. DNA sequences consist of four letters (also called bases): A, T, C, and G. The DNA sequences of humans have approximately 4 billion bases (4 x 10). How many possible DNA sequences of length 4 billion are there?
DNA sequences are made up of 4 bases: A (adenine), C (cytosine), G (guanine), or T...
DNA sequences are made up of 4 bases: A (adenine), C (cytosine), G (guanine), or T (thymine). How many 8-element DNA sequences a) Do not end with C or T? b) Start with A and ends with T? c) Start with G? d) Contain only G or C?
The observation that in any DNA sample, A T and G C A. DNase sequencing An...
The observation that in any DNA sample, A T and G C A. DNase sequencing An analytical method that determines which segments of DNA are bound by a particular B. Chargaff's rule protein factor, such as a transcription factor C. ChIP sequencing D. Euchromatin E. Histone acetylation F. major groove - # Areas associated with a eukaryotic gene that are where most DNA methylation occurs. # An analytical technique that involves a small slide or chip with many segments of...
Question 2. True / False (10 marks) Tor F The DNA sequences shown below are complementary...
Question 2. True / False (10 marks) Tor F The DNA sequences shown below are complementary to each other. AATTGCTACGTT AATTGCTACGTT I or F T or F b. Primase carries polymerase activity. c. In prokaryotes, RNA polymerases synthesize RNA at a lower rate than DNA polymerases because it takes time for RNA polymerase to perform proofreading. d. Reverse transcriptase activity is observed in DNA polymerase I in prokaryotes. The base in the following nucleotide is adenine. This nucleotide can be...
III. Answer the quest a 1. a. Use the lined up homologous DNA base sequences below...
III. Answer the quest a 1. a. Use the lined up homologous DNA base sequences below to determine the mutations required to reconstruct the phylogeny of Taxa R, S & T (see answer sheet). DNA sites 1 2 3 4 a. Label the tree with the appropriate mutations using hash marks, 2 pts. Ancestor AACG b. Why is this the best phylogeny of the 3 taxa? I pt. Taxon R GCTG c. Which two taxa form a monophyletic group? 1...
based on the given graph how would these be answered? sa sequence from a sheep PCR...
based on the given graph how would these be answered?
sa sequence from a sheep PCR product. The template used was genomic DNA and the forward and se primers were designed to amplify partofthe priongene, which encodes the causative agentot scrapie. is black;Cisblue; Ais green: Tisred. The forward primerwasusedasasequencing primer. TheNattheten ow means that the computer can't decide which nucleotide is at that position in the sequence because there are two peaks (red=T and blue-C) that are the same height....
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its KEY corresponding peak. - 2pts And memes 1. Wallet 2 banan sam hesalesanan Amalan 3. 4. 12 V Α' Α' Aav po e te AL А. ab x *² ADA Style 5. Are the sequences above identical to the DNA Template? Why or why not? -1pt
Using the key provided (in the first photo on the right hand
side) interpret the nucleotide base sequences in the gour
electropherograms below. Write each base below its corresponding
peak.
Also, are the sequences above idential to the DNA template? Why or
why not?
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its corresponding peak. - 2pts KEY...
Directions: Using the key provided, interpret the nucleotide base ssion 1) sequences in the four electropherograms below. Write each base below its corresponding peaked interpret the the 2pts mucodebase sunces in the four electroplerograms below. Write in each base below its corresponding peak KEY banana Selebobolanie brake 5. Are the sequences above identical to the DNA Template? Why or why not? -1pt 6. What are the five main components of a PCR-based dye terminator reaction? -2.5pts
Problem 1. DNA sequences consist of four letters (also called bases): A, T, C, and G. The DNA sequences of humans have approximately 4 billion bases (4 x 10). How many possible DNA sequences of length 4 billion are there?
The observation that in any DNA sample, A T and G C A. DNase sequencing An analytical method that determines which segments of DNA are bound by a particular B. Chargaff's rule protein factor, such as a transcription factor C. ChIP sequencing D. Euchromatin E. Histone acetylation F. major groove - # Areas associated with a eukaryotic gene that are where most DNA methylation occurs. # An analytical technique that involves a small slide or chip with many segments of...
Question 2. True / False (10 marks) Tor F The DNA sequences shown below are complementary to each other. AATTGCTACGTT AATTGCTACGTT I or F T or F b. Primase carries polymerase activity. c. In prokaryotes, RNA polymerases synthesize RNA at a lower rate than DNA polymerases because it takes time for RNA polymerase to perform proofreading. d. Reverse transcriptase activity is observed in DNA polymerase I in prokaryotes. The base in the following nucleotide is adenine. This nucleotide can be...
III. Answer the quest a 1. a. Use the lined up homologous DNA base sequences below to determine the mutations required to reconstruct the phylogeny of Taxa R, S & T (see answer sheet). DNA sites 1 2 3 4 a. Label the tree with the appropriate mutations using hash marks, 2 pts. Ancestor AACG b. Why is this the best phylogeny of the 3 taxa? I pt. Taxon R GCTG c. Which two taxa form a monophyletic group? 1...
based on the given graph how would these be answered?
sa sequence from a sheep PCR product. The template used was genomic DNA and the forward and se primers were designed to amplify partofthe priongene, which encodes the causative agentot scrapie. is black;Cisblue; Ais green: Tisred. The forward primerwasusedasasequencing primer. TheNattheten ow means that the computer can't decide which nucleotide is at that position in the sequence because there are two peaks (red=T and blue-C) that are the same height....
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