Homework Answers
Que 6 What are the five main components of PCR based dye terminator reaction?
Ans The five main components of PCR- based dye terminator reaction are-
DNA template
Taq DNA polymerase,
oligonucleotide primers,
nucleotides,
terminator nucleotides.
NOTE-Ques5 based on peak which hasn't clear description.
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Directions: Using the key provided, interpret the nucleotide base ssion 1) sequences in the four electropherograms...
Using the key provided (in the first photo on the right hand side) interpret the nucleotide...
Using the key provided (in the first photo on the right hand
side) interpret the nucleotide base sequences in the gour
electropherograms below. Write each base below its corresponding
peak.
Also, are the sequences above idential to the DNA template? Why or
why not?
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its corresponding peak. - 2pts KEY...
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided,...
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its KEY corresponding peak. - 2pts And memes 1. Wallet 2 banan sam hesalesanan Amalan 3. 4. 12 V Α' Α' Aav po e te AL А. ab x *² ADA Style 5. Are the sequences above identical to the DNA Template? Why or why not? -1pt
Sequences from Picture: 1. A C T T C T T G C C A C...
Sequences from Picture:
1. A C T T C T T G C C A C T G G T T G C T G C A A
2. G A G T A A T A C A G A G C G A G A G G A C G A C A
3. A T T G C T C C A G A T T G C A A
4. T C T A C T...
part a) part b) Consider the activity you have just completed. Compare your results for electropherogram...
part a)
part b)
Consider the activity you have just completed. Compare your
results for electropherogram #1 to those for electropherogram #4.
Could sequence data be accurately read from electropherogram #4?
Why or why not?
Directions: Using the key provided, interpret the nucleotide base sequences in the four electrophorograms below. Write each base below its corresponding peak. - 2pts KEY ܫܢܡܠܐܠܕܬܐ bana ܚܬܐܬܠܘܐܢܩܠܣܬܬܠܘ
S-CGT-3 GTG 3. Write in the following sequences to depict them hybridizing/annealing to complementary and antiparallel...
S-CGT-3 GTG 3. Write in the following sequences to depict them hybridizing/annealing to complementary and antiparallel sequence in the exposed nucleotide chains. 5'-GTG-3 5-CGT-3 5'-ACG-3' | 5 -CAC-3" 5'-AAT[CGTATCAGCAGCAGTG|ACT-3 -3'-TTALGCATAGTCGTCGTCATGA-5'- 3. Two of the four above sequences can be used together as a "primer pair" to PCR amplify the bracketed sequence. In order to determine which two will work, recall that new polynucleotide chains can only be added to on the 3'end. Draw an arrow from the 3' end of...
strand(s) and the RNA molecule is made up of strand(s). The DNA molecule is made up...
strand(s) and the RNA molecule is made up of strand(s). The DNA molecule is made up of O a. 4; 2 Ob. 1; 1 O c. 2; 1 O d. 1; 2 O e. 2; 4 QUESTION 45 The formation of large molecules from small subunits is known as what kind of reaction? O a reduction O b.condensation Oc decarboxylation d. hydrolysis O e. oxidation What is formed when an atom loses or gains an electron? O a. ion b....
Using the key provided (in the first photo on the right hand
side) interpret the nucleotide base sequences in the gour
electropherograms below. Write each base below its corresponding
peak.
Also, are the sequences above idential to the DNA template? Why or
why not?
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its corresponding peak. - 2pts KEY...
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its KEY corresponding peak. - 2pts And memes 1. Wallet 2 banan sam hesalesanan Amalan 3. 4. 12 V Α' Α' Aav po e te AL А. ab x *² ADA Style 5. Are the sequences above identical to the DNA Template? Why or why not? -1pt
Sequences from Picture:
1. A C T T C T T G C C A C T G G T T G C T G C A A
2. G A G T A A T A C A G A G C G A G A G G A C G A C A
3. A T T G C T C C A G A T T G C A A
4. T C T A C T...
part a)
part b)
Consider the activity you have just completed. Compare your
results for electropherogram #1 to those for electropherogram #4.
Could sequence data be accurately read from electropherogram #4?
Why or why not?
Directions: Using the key provided, interpret the nucleotide base sequences in the four electrophorograms below. Write each base below its corresponding peak. - 2pts KEY ܫܢܡܠܐܠܕܬܐ bana ܚܬܐܬܠܘܐܢܩܠܣܬܬܠܘ
S-CGT-3 GTG 3. Write in the following sequences to depict them hybridizing/annealing to complementary and antiparallel sequence in the exposed nucleotide chains. 5'-GTG-3 5-CGT-3 5'-ACG-3' | 5 -CAC-3" 5'-AAT[CGTATCAGCAGCAGTG|ACT-3 -3'-TTALGCATAGTCGTCGTCATGA-5'- 3. Two of the four above sequences can be used together as a "primer pair" to PCR amplify the bracketed sequence. In order to determine which two will work, recall that new polynucleotide chains can only be added to on the 3'end. Draw an arrow from the 3' end of...
strand(s) and the RNA molecule is made up of strand(s). The DNA molecule is made up of O a. 4; 2 Ob. 1; 1 O c. 2; 1 O d. 1; 2 O e. 2; 4 QUESTION 45 The formation of large molecules from small subunits is known as what kind of reaction? O a reduction O b.condensation Oc decarboxylation d. hydrolysis O e. oxidation What is formed when an atom loses or gains an electron? O a. ion b....
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