Question

part a)

Directions: Using the key provided, interpret the nucleotide base sequences in the four electrophorograms below. Write each base below its corresponding peak. - 2pts KEY

part b)

Consider the activity you have just completed. Compare your results for electropherogram #1 to those for electropherogram #4. Could sequence data be accurately read from electropherogram #4? Why or why not?

Answer 1

Ans :

Part a)

• Figure 1 - Electropherogram with high quality sequence (Sharp peaks with little background )

A C T T C T T G C C A C T G G T T G C T G C A A G

• Figure 2- Electropherogram with mobility errors (Overlapping)

G A G T A A T A C A G A G C G A G A G G A C G A C A

• Figure 3 - Electropherogram that has spread (Bands are spread out and become irresolvable)- N represents no band

A T T T G C T C C N C A A N G A A T T G C G A A A

• Figure 4 - Electropherogram that demonstrates a lack of extension products .i.e no bands(N represents no band)

C T A N T T C T T A N A C T A N A N C N A N A G C N N

Part b) No. It is because in electropherogram #4 there are too many 'N' nucleotides in the resulting sequence .The causes can be because of low quality, low concentration or degraded primer used. Another cause will be not adding a reagent or malfunction by the 3700 DNA Analyzer.

Solution for this problem is by repeating the reaction, purifying the template or redesigning the primer.