Which of these features are required to select recombinant bacteria? a plasmid with an antibiotic resistance...
Which of these features are required to select recombinant bacteria?
a plasmid with an antibiotic resistance gene
a plasmid with a reporter gene
a plasmid with an origin of replkication
a plasmid that is expressing the gene of interest
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Which of these features are required to select recombinant
bacteria?
a plasmid with an antibiotic resistance...
Antibiotic selection allows you to determine which bacteria contain the plasmid of interest, but do not...
Antibiotic selection allows you to determine which bacteria contain the plasmid of interest, but do not necessarily tell you if the plasmid has taken up the recombinant DNA and contains your gene of interest. Which gene, which spans the multiple cloning site (MCS), allows you to screen for plasmids that contain your insert? A. β-lactamase (bla) B. Ori C. lacZ D. X-gal
Which of the following is the easiest was for bacteria to develop antibiotic resistance? Select one:...
Which of the following is the easiest was for bacteria to develop antibiotic resistance? Select one: a. Mutation of a gene that regulates antibiotic resistance. b. Generate a mutation to alter the target protein. c. Acquire drug resistance genes via lateral gene transfer. d. Modify the antibiotic once it cross the bactieral membrane e. None of the choices.
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation....
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation. B. Recombinant DNA molecules usually contain a DNA fragment inserted into a bacterial vector. C. To insert a gene of interest into a vector, the gene of interest and the vector must be digested with different restriction enzymes D. Transformed bacteria are plated onto media containing the appropriate antibiotic and only bacteria containing the plasmid with antibiotic resistance will grow.
Assume there is a plasmid, pAGG1 that carries two genes, geneA encoding the antibiotic resistance gene...
Assume there is a plasmid, pAGG1 that carries two genes, geneA encoding the antibiotic resistance gene to select the plasmid and geneX which is your gene of interest. Each of the two genes is about 0.6kb encoding proteins of about 200 amino acids. Question: Discuss what limitations there are in mutagenizing the entire plasmid to saturate geneX, when would you select a whole gene method to mutagenize geneX versus a site directed method?
Recently an antibiotic resistant strain of bacteria was found to possess a plasmid containing a unique...
Recently an antibiotic resistant strain of bacteria was found to possess a plasmid containing a unique drug resistance gene. Scientists and physicians are extremely concerned about this discovery since this drug resistance gene is usually only found as a part of the bacterial chromosome (nucleoid). In 1 bullet point --Explain the differences between chromosomal DNA (nucleoid) and plasmids. In 2 bullet points -- Explain why the medical community is so concerned about the the discovery of this particular antibiotic resistant...
In regard to biotechnology application, what is the purpose of using a recombinant plasmid that encodes...
In regard to biotechnology application, what is the purpose of using a recombinant plasmid that encodes an ampicillin resistance gene? Choose one: O A. The product of the ampicillin gene is ampicillin, and the ampicillin confers better survival and more cell reproduction O B. Ampicillin is an antibiotic so this protects the researcher from infection. O C. This allows the researcher to use ampicillin in the culture and select for cells that acquired the plasmid. O D. This allows the...
A plasmid containing recombinant DNA is introduced into a bacteria. This plasmid is cloned during... a....
A plasmid containing recombinant DNA is introduced into a bacteria. This plasmid is cloned during... a. metaphase I b. binary fission c. the S phase of interphase d. mitosis
Notes 1. What are the key features of a plasmid. How will you exploit those features...
Notes 1. What are the key features of a plasmid. How will you exploit those features to make a recombinant protein? 2. You want to express a protein that makes cheese with jalapeño flavor. How will you do it? 3. How do bacteria acquire Antibiotic resistance? 4. How will you treat a bacterial disease using bacteriophages? 5. How does blue/white screening work? How will you exploit this to clone genes of interest? 6. How do antibiotics and antibiotic resistance influence...
When transforming bacteria in the lab, you co-transform an antibiotic resistance gene along with your gene...
When transforming bacteria in the lab, you co-transform an antibiotic resistance gene along with your gene of interest. By plating those bacteria on antibiotic plates, what principle are you relying on? Evolution O Counter-selection Adaptation Selection
Explain briefly (and within the context of the plasmid you just created) the role of each...
Explain briefly (and within the context of the plasmid you just created) the role of each of the 10 elements that are in bold it needs a “ColE1 origin” (E. coli origin of replication) · it needs a “2μ ori” (yeast origin of replication) · a AmpR gene (coding for the bacterial resistance against the antibiotic ampicillin) and its promoter. · a yeast gene coding for the synthesis of uracil and its promoter. · a yeast gene coding for the...
In regard to biotechnology application, what is the purpose of using a recombinant plasmid that encodes an ampicillin resistance gene? Choose one: O A. The product of the ampicillin gene is ampicillin, and the ampicillin confers better survival and more cell reproduction O B. Ampicillin is an antibiotic so this protects the researcher from infection. O C. This allows the researcher to use ampicillin in the culture and select for cells that acquired the plasmid. O D. This allows the...
Notes 1. What are the key features of a plasmid. How will you exploit those features to make a recombinant protein? 2. You want to express a protein that makes cheese with jalapeño flavor. How will you do it? 3. How do bacteria acquire Antibiotic resistance? 4. How will you treat a bacterial disease using bacteriophages? 5. How does blue/white screening work? How will you exploit this to clone genes of interest? 6. How do antibiotics and antibiotic resistance influence...
When transforming bacteria in the lab, you co-transform an antibiotic resistance gene along with your gene of interest. By plating those bacteria on antibiotic plates, what principle are you relying on? Evolution O Counter-selection Adaptation Selection
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