Which method requires the experimental construction of two fusion proteins to assess protein–protein interactions? yeast two-hybrid...
Which method requires the experimental construction of two fusion proteins to assess protein–protein interactions?
yeast two-hybrid system
Western blot
Northern blot
immunoprecipitation
Homework Answers
Option D, immunoprecipitation
In Immunoprecipitation (IP) antigen is separated from a mixture based on the antigen-antibody interaction. Antigen -antibody complexes is formed by adding antibody to antigen mixture. The complex is incubated against primary antibody/ protein A/G-coated so that it absorbs the complex. Later, antigen is derived from the mixture through acidic solution.
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Which method requires the experimental construction of two
fusion proteins to assess protein–protein interactions?
yeast two-hybrid...
The yeast two hybrid system, which is used for detecting interacting gene products, is dependent on...
The yeast two hybrid system, which is used for detecting interacting gene products, is dependent on which of the following: Choose one answer: A) activation of DNA polymerase by the nearby binding of interacting protein complexes B) having a promoter that responds directly to one of the two proteins whose interactions is being measured C) the interaction of DNA segments encoding proteins of interest . D) the stimulation of transcription by protein-protein interaction which leads to the formation of a...
The lab in which you work wants to use a yeast two-hybrid (Y2H) assay to identify...
The lab in which you work wants to use a yeast two-hybrid (Y2H) assay to identify binding partners (prey) for a newly discovered yeast protein (bait). Your lab's Y2H system uses the Gal4 transcription factor. You begin by testing the Y2H system with a pair of proteins that exhibit a strong, direct binding interaction in several in vitro binding assays. Unfortunately, you do not see any surviving colonies. You know that the commercial Y2H system you used in your experiment...
Proteins: BSA, yeast ADH, ovalbumin, lysozyme . Discuss how you were able to determine which protein...
Proteins: BSA, yeast ADH, ovalbumin, lysozyme
. Discuss how you were able to determine which
protein was in which lane. You will have to do some
online research to find the molecular weight of each protein,
whether it is a dimer, etc. For at least one of the proteins, there
is some variability regarding the molecular weight, but the range
should still allow you to determine which lane each of the proteins
is in. For each lane, pay attention to...
Word bank: cDNA Cre CRISPR FISH LoxP Microarray RFLP RNAi Yeast Two-Hybrid 1) This enzyme binds...
Word bank: cDNA Cre CRISPR FISH LoxP Microarray RFLP RNAi Yeast Two-Hybrid 1) This enzyme binds loxP sites and catalyzes a recombination event. 2) Two 13-bp inverted repeats separated by an 8-bp spacer that can be used to recombine DNA in vivo in transgenic organisms. 3) A gene system using Gal4 and Gal80 to detect protein-protein interactions. 4) This technique allows you to fluorescently tag certain proteins and see them in whole tissue to determine the pattern of expression. 5)...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
Proteins: BSA, yeast ADH, ovalbumin, lysozyme
. Discuss how you were able to determine which
protein was in which lane. You will have to do some
online research to find the molecular weight of each protein,
whether it is a dimer, etc. For at least one of the proteins, there
is some variability regarding the molecular weight, but the range
should still allow you to determine which lane each of the proteins
is in. For each lane, pay attention to...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
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