Question

The yeast two hybrid system, which is used for detecting interacting gene products, is dependent on which of the following: Choose one answer:

A) activation of DNA polymerase by the nearby binding of interacting protein complexes

B) having a promoter that responds directly to one of the two proteins whose interactions is being measured

C) the interaction of DNA segments encoding proteins of interest .

D) the stimulation of transcription by protein-protein interaction which leads to the formation of a Gal4p DNA binding and activation domain complex.

Answer 1

Answer: D) the stimulation of transcription by protein-protein interaction which leads to the formation of a Gal4p DNA binding and activation domain complex.

Explanation:

Yeast two-hybrid system identify protein–protein interactions and protein–DNA interactions with the help of binding between two proteins or DNA and protein respectively.

Yeast two-hybrid systems involves interaction of two partners: DNA-binding domain of Gal4 and activation domain. It produces a functional transcription activator.

Gal4 transcription factor gene is required for transcription of the reporter gene which is LacZ.