How many different combinations are possible for a 10 bp DNA primer?
Label all of the enzymes in the diagram below. Also, be sure to label the leading and lagging strands and the Okazaki fragments. (0.5 pt.)
Homework Answers
1. Total # bases = 4 (ATGC)
At every position, one out of 4 bases will come. There are 10 such positions.
So, # combinations = 4^10 = 1048576
2.
Helicase - breaks down hydrogen bonds present between two strands of DNA and converts them into single strands of DNA
Single stranded binding proteins - maintains single stranded molecule of DNA in single standard form
Toposiomerase - relaxes supercoiling which occurs ahead of the replication
Primase - synthesizes RNA primer which provide free hydroxyl group to DNA polymerase 3
DNA polymerase 3 - adds deoxyribonucleotides to the growing daughter DNA molecule
DNA polymerase 1 - removes RNA primer and fills in the gap between adjacent okazaki fragments
Ligase - formation of phosphodiester bond between adjacent okazaki fragments to form a single continuous molecule of DNA
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