You calculated the live cell concentration to be 1.5 x 10^6 cells/mL. what is the volume...
You calculated the live cell concentration to be 1.5 x 10^6 cells/mL.
what is the volume of cell suspension needed to seed a T-75 flask with 7.5 x 10^5 cells in 10 mLs of media.
Homework Answers
Given cell suspension concentration = 1.5 X 106
cells/mL
Required cell count = 7.5 X 105 cells
1 mL ------> 1.5 X 106 cells
? mL ------> 7.5 X 105 cells
= 750000/1500000
= 0.5 mL
Take 0.5 mL of the original cell suspension and add 9.5 mL of the
media to obtain 7.5 X 105 cells in 10 mL media
solution
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