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You calculated the live cell concentration to be 1.5 x 10^6 cells/mL. what is the volume...

You calculated the live cell concentration to be 1.5 x 10^6 cells/mL.

what is the volume of cell suspension needed to seed a T-75 flask with 7.5 x 10^5 cells in 10 mLs of media.

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Answer #1

Given cell suspension concentration = 1.5 X 106 cells/mL
Required cell count = 7.5 X 105 cells
1 mL ------> 1.5 X 106 cells
? mL ------> 7.5 X 105 cells

= 750000/1500000
= 0.5 mL

Take 0.5 mL of the original cell suspension and add 9.5 mL of the media to obtain 7.5 X 105 cells in 10 mL media solution

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