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  What kind of experiment would allow you to test that the posterior cytoplasm can cause the...

  What kind of experiment would allow you to test that the posterior cytoplasm can cause the formation of germ cells?

How can kinesin and microtubules contribute to the formation of the germ cells?

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Q-1):

  • In Drosophila fly, the experiment has done. The fruit fly provides a powerful experimental model system for the germ cell formation.
  • The role of germplasm in germ cell formation and function has been well demonstrated.
  • In Drosophila. Here, the germplasm is localized at the posterior of the early embryo, thereby restricting the formation to the posterior pole.
  • In these germ cells are formed from a specialised, maternally provided cytoplasm that is sequestered at the posterior pole of the oocyte during oogenesis.
  • Oskar protein and at least three other RNA binding proteins, Vasa, Tudor, Aubergine are essential for germplasm assembly, including large ribosome rich structures, the polar granules
  • Which harbour germline-specific RNAs and proteins.localisation of Oskar RNA and protein and thus germ plasm assembly is regulated by microtubule directed RNA transport, by RNA slicing and transitional control.

Q-2.

  • Kinesins and Microtubules play an important role in the formation of the germ cell.
  • Kinesins and Microtubule help in the transport of germ cell compartments.
  • Kinesins are motor proteins that transport their cargos along microtubules in an ATP -dependent manner.
  • Germplasm assembly occurs during oogenesis, initiated by the kinesin- dependent localization and subsequent translation of Oskar mRNA at the oocyte posterior.
  • A series of unconventional microtubule-organizing centres play a fundamental role during germ cell formation.
  • The effect of disrupting germplasm transport is by taking advantage of mutations in centrosome components including Aur kinase.

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