Bacteria growth is controlled by both sterilization and sanitization. Below list 3 physical sterilization methods, 3 chemical sterilization methods, and 3 chemical sanitization methods. Include the mechanism used by sanitization chemicals to control bacterial growth.
Sterilization include discontamination by the destruction of microorganisms including its spores through use of toxic chemicals, high temperature or through radiatiosns.
Three physical sterilization methods include 1) Boiling 2) Autoclaving 3) Pasteurization
Three chemical sterilization method include use of 1) Formaldehyde 2) Hydrogen peroxide 3) Glutaraldehyde
Sanitization reduces the number of microorganisms to a safe level by the destruction of the microbes on wounds, clothes, hard surfaces etc thus reducing the chances of getting sick.
Three most commonly used chemical sanitizers are 1)Chlorine based cleaners, 2)Quaternary ammonia 3) Iodine sanitizers
Mode of action of these chemical sanitizer is by dissolving the outer membrane of the bacteria and make them explode. These chemicals have water loving hydroxyl group which disrupts the protein and lipid molecules that make up the bacterial membrane and makes the outer coat falls apart resulting in lysis of the membrane
Bacteria growth is controlled by both sterilization and sanitization. Below list 3 physical sterilization methods, 3...
Background
Questions
Bacteria in the environment must be controlled to reduce the chance of spreading the bacteria and possible infectious disease. There are many methods of controlling bacterial numbers; heat, chemicals, drugs and radiation are a few. Each control method targets something within the cell either structurally or metabolically that leads to cellular death. Microbes are most vulnerable during the exponential growth phase, when there is a high level of metabolism and cellular structures are being created. Many control methods...
List and describe the phases of the bacteria growth with regards to: a) Number of cells; b)”Tooling”; c) generation time; d) viability; e) mortality, f) pH changes in media; g) waste production List three (3) instruments often used to detect the growth of bacteria. How are these instruments used to estimate the growth of microorganisms? Discuss the techniques that are frequently employed to detect/estimate bacteria growth. Explain the advantages and weaknesses of these methods.
Lethal mutations in the exposed microbes. you up uptr 3. Matching h. Alcohols _Antisepsis a) Stops the microbe from growing %) They bioaccumulate and can be harmful to humans c) Chlorine used for less than 30 minutes J. Cidal Disinfection hater treatment d) (a) Todine Halogens og Bens chlorine Fluorine b. Heavy Metals Peroxygens-Hyde la Phenolics Tobish List Conic acid d e) Includes a chemical that can kill and destroy everything, including naked viruses f Some microbes are catalase 2.0...
3) We used two methods, turbidity and spread plates, to determine bacterial growth. What is a disadvantage of turbidity over spread plate? 4) We used two methods, turbidity and spread plates, to determine bacterial growth. What is a disadvantage of spread plate over turbidity?
Chapters 7, 8, 9 - Bacterial Growth & Metabolism (some chapter sections will be covered in lab) Prerequisite: Basic catabolic pathways (respiration and fermentation) and anabolic reactions (photosynthesis) BACTERIAL GROWTH AND CONTROL- Some of these topics will be covered in greater detail during lab Environmental Growth Factors 1. Discuss the specific role of quorum sensing in biofilm formation Control of Microbial Growth 2. Describe the methods used to control microbial growth 3. List the types of antibiotics that inhibit (a)...
MODULE 15 EFFECTS OF DISINFECTANTS AND ANTISEPTICS OBJECTIVES Be familiar or be able to describe 1) Antimicrobial substances: Chemical and biological 2) Bacteriocidal versus Bacteriostatic chemicals 3) Sterilization versus disinfection by chemical antimicrobials 4) Difference between disinfectants, antisepties, and antibiotics in terms of a. Antimicrobial activity b. Where they would be used c. Role of organic matter in their effectiveness d. Differential effects on Gram positive and Gram negative bacteria. 5) Phenol coefficient test 6) Disk Diffusion method of assessing...
and Lab Exer n this experiment you will evaluate effect of UV light on bacterial growth. UV light affects growth by interfering with DNA, resulting in thymine dimer formation. The dimerization is confined within adjacent thymine residues on same strand (intrastrand ). These dimmer lesions have consequence on DNA functions including interference in DNA replication, transcription etc, so UV exposure of cells can lead to lethal effects for bacterial multiplications. This forms the basis for UV method to control bacterial...
Below
are results of the Ames Test that was conducted to determine
whether a compound called sodium azide could be useful for causing
detrimental mutations in bacteria, thus preventing bacterial growth
on surgical tools. To test the mutagenicity of sodium azide, the
Ames test was carried out. The bacterial species Salmonella
typhimurium requires Histidine to grow and was plated on minimum
growth plates lacking histidine. Bacteria plated on the control
plate had only buffer added, while bacteria on the treatment...
1)An eosin methylene blue (EMB) agar plate was inoculated with four different species of bacteria. Below are the colony growth after inoculation. Which shows a vigorous lactose fermenter? A) A brown media with an oblong culture of bacteria. The colony shows vigorous growth and is the same color as the media. B) A brown media with an oblong colony of bacteria that experiences weak growth. The color of the media is visible through the translucent white color of the bacterial...
35. Bacteriophage Research: Below is an example of experimental results from a bacteriophage one-step growth curve. This experiment was conducted to compare the number of bacteriophages produced in the presence or absence of a newly identified natural remedy nicknamed "Bacteriophage-Be-Gone" that has been shown by others to reduce virus production. Please analyze the results and answer the questions below. Number of bacteriophages 30 60 180 210 240 90 120 150 time (min) a. (10 points) Please label (on the graph)...