2) The DNA from crime scene and those of the suspects are taken and RFLP analysis is done to find out a pattern of the RFLP, as they vary in different individuals. If the pattern of RFLP from the crime scene matches with the RFLP of any of the suspect, this proves that , that particular suspect was at the crime scene. In the given figure the RFLP pattern after gel electrophoresis from the crime scene matches with the RFLP pattern of that of suspect 2, while for suspect 1 and 3, the RFLP pattern is quite different than that obtained from the crime scene. So, it can be said that suspect2 was at the crime scene.
2. Gel analysis practice 1) Label in the below picture and indicate the size of each...
3. PCR analysis practice One 3) If you collect samples from crime scene and extract DNA out of each sample. Describe the technologies used to get the below results. 4) Identify which suspect is at the crime scene 5) Among suspect 1-3, who is homozygous for the tested locus and who is heterozygous? DNA from crime scene DNA ladder suspect DNA #1 #2 #3 500 bp 400 bp 300 bp 200 bp 100 bp — I 4. PCR Practice two...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
7. Sketch your gel in the space below. Clearly label the samples present in each lane. Also, label the bands that correspond to the 1000 bp, 700 bp, 500 bp, 200 bp, and 100 bp in the DNA standard lane. bp ooobp ook 8. What are the genotypes of each lab member for the Alu insert in the PV92 region? Studunt : /+ Studunt 2:-/- 9. Which students contain the Alu insert? How do you know? studentI bandl corresponds to41...
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the horitzontal. I need help figuring out where to start and what to do. Please help! The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
HELP PLEASE! at least with some examples. size of dna ladder- 1.8cm We were unable to transcribe this imageTable 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA band on the gel by interpolating values from your standard curve. There may be multiple bands per lane.For EACH band, identify size (interpolated from the standard curve you constructed), identity, shape, and topology DISTANCE EACH BAND IN THE LANE H AS MIGRATED SIzE OF...
b. Make a TABLE 1 with distance migrated (cm or mm) vs size (in bp) of the 1 kb Ladder standard bands. [See Example) Measure distance migrated for each size standard band on gel. (5 pts) DNA Standard Undigested Pstl+Sac!! Pstl+Stul Pstl+Sacll+Stut Pst! BOS S Kilobases Mass (ng) - 10.0 -80 (bp) 3,300 3,000 2.900 2,700 2,500 0 CC 2,700 2,400 2,000 1,800 1,750 1,800 1,600 1,400 1.300 1,250 1,000 850 800 750 700 650 600 550 -0.5 42 500
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel electrophoresis, the DNA fragments can be separated on a gel, based on their lengths. In order to see the fragments, a stain is typically added to the gel. The size of each fragment can be determined by comparing each one to a DNA molecular weight marker of known size. Below is a map of pBR22 plasmid. The position and base pair number of the...
Hi can someone help me understand part C and why the drawn in red lines are where they are. Basically from the bp given how can I go back to cm so I can drawn them into the picture provided? Do not need help with A or B. The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes...
Please complete tables for forensic biology lab using the gel image as reference. If you could complete the whole table that would be much appreciated, however I do not expect anyone to do that much work for me. If you could just complete a few rows on each one and show me how you got there, then I'm sure I could complete the rest of it (I'm just confused on how to calculate the distance in cm) My teacher gave...
Marginal Cost Average Variable Cost Average Total Cost Quantity of Exercise Machines 1 2 3 4 5 6 7 8 9 10 $200 $400 $600 $800 $1,000 $1,200 $1,400 $1,600 $1,800 $2,000 $200 $300 $400 $500 $600 $700 $800 $900 $1000 $1100 $1200 $800 $733.33 $750 $800 $866.67 $942.86 $1025 $1111.11 $1200 The above table shows the costs of a small manufacturer producing different quantities of exercise machines. If the exercise machine market is perfectly competitive and exercise machines cost...