Can this standard curve for yeast cells be used for E.coli cells why/why not?
Homework Answers
No,standardcurve for yeast cannot be used for E.coli because the absorbance values will be different in each case.
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Can this standard curve for yeast cells be used for
E.coli cells why/why not?
- conc...
What is the Slope and the Y-intercept? - conc of dilu Trendline for conc of dilu...
What is the Slope and the
Y-intercept?
- conc of dilu Trendline for conc of dilu R2 = 1 1.00 0.75 Concentration 0.50 0.25 0.00 0.2 0.3 0.4 0.5 0.6 0.7 od
What is the slope and Y-intercept? - conc of dilu Trendline for conc of dilu R2...
What is the slope and Y-intercept?
- conc of dilu Trendline for conc of dilu R2 = 1 1.00 0.75 Concentration 0.50 0.25 0.00 0.2 0.3 0.4 0.5 0.6 0.7 od
6. Which graphs can be used to determine the amount of chemical denaturant needed to unfold...
6. Which graphs can be used to determine the amount of
chemical denaturant needed to unfold half of the protein molecules
in a sample? select all apply
7. Which graphs can be used to determine whether or not
binding a ligand influences binding of additional ligands? select
all that apply
8. Which graphs can be used to hypothesize a mechanism of
reversible inhibition? select all tgat apply
fraction bound th fraction unfolded 0.7 0 0.1 0.2 0.3 0.4 0.5 0.6...
please help with ALL questions thank you 1. Using excel, plot your standard curve based on...
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
1. Which graph(s) can be used to estimate a Km for an enzymatic reaction? select all...
1. Which graph(s) can be used to estimate a Km for an
enzymatic reaction? select all that apply
2. Which graph(s) can be used to calculate an association
constant for a protein binding its ligand? select all that
apply
3. Which graph(s) can be used to estimate protein stability
and to understand folding thermodynamics? select all that
apply
4. Which graph(s) can be used to estimate the equilibrium
constant for the dissociation of a protein-ligand complex? select
all that apply...
1- What is a standard curve and how is it used in biochemistry? 2- Explain how...
1- What is a standard curve and how is it used in biochemistry? 2- Explain how the Lambert-Beer equation is applied in this experiment. 3- Why is a spectrophotometer used to obtain data? 4- Based on the experimental data provided, estimate the amount of amino acid in the given unknown solution by Ninhydrin method. SI No. Amount of amino acid [pg) OD at 570nm 1 2 3 4 5 6 Volume of standard amino acid solution (ml) Blank 0.2 0.4...
1. Which graphs can be used to estimate a Km for an enzymatic reaction? select all...
1. Which graphs can be used to estimate a Km for an enzymatic
reaction? select all that apply
2. Which graphs can be used to calculate an association
constant for a protein binding its ligand? select all that
apply
3. Which graphs can be used to estimate protein stability and
to understand folding thermodynamics? select all that apply
4. Which graphs can be used to estimate the equilibrium
constant for the dissociation of a protein-ligand complex? select
all that apply...
Please answer what’s required from the following question correctly. The curve in the following diagram represents...
Please answer what’s required from the following question
correctly.
The curve in the following diagram represents a traverse across the center of a roughly equidi- mensional ore body. The anomaly due to the ore body is obscured by a strong regional anoma- ly. Remove the regional anomaly and then evaluate the anomaly due to the ore body. which has a density contrast of 0.75 g/cm. (Graph values are supplied in the table following the graph.) 0.00 -0.25 0.50 -0.75 0.0...
The following are quality control data for a manufacturing process at Kensport Chemical Company. The data...
The following are quality control data for a manufacturing process at Kensport Chemical Company. The data show the temperature in degrees centigrade at five points in time during a manufacturing cycle. Sample x R 1 95.72 1.0 2 95.24 0.9 3 95.18 0.9 4 95.48 0.4 5 95.46 0.5 6 95.32 1.1 7 95.40 1.0 8 95.44 0.3 9 95.08 0.2 9 10 10 95.50 0.6 11 95.80 0.6 12 95.22 0.2 13 95.60 1.3 14 95.22 0.5 15 95.04...
A saturated liquid feed with a composition of 45 mole-% benzene and 55 mole-% toluene is...
A saturated liquid feed with a composition of 45 mole-% benzene and 55 mole-% toluene is distilled in a plate column at the rate of 250 kmol/h. Overhead product contains 97 mole-% benzene and the bottom product contains 5 mole-% benzene. A total condenser and a partial reboiler are used. The reflux ratio employed is 1.5 times the minimum, RD 1.5 RDmin Find the number of theoretical plates required for this separation, the heat duties of the condenser and reboiler....
What is the Slope and the
Y-intercept?
- conc of dilu Trendline for conc of dilu R2 = 1 1.00 0.75 Concentration 0.50 0.25 0.00 0.2 0.3 0.4 0.5 0.6 0.7 od
What is the slope and Y-intercept?
- conc of dilu Trendline for conc of dilu R2 = 1 1.00 0.75 Concentration 0.50 0.25 0.00 0.2 0.3 0.4 0.5 0.6 0.7 od
6. Which graphs can be used to determine the amount of
chemical denaturant needed to unfold half of the protein molecules
in a sample? select all apply
7. Which graphs can be used to determine whether or not
binding a ligand influences binding of additional ligands? select
all that apply
8. Which graphs can be used to hypothesize a mechanism of
reversible inhibition? select all tgat apply
fraction bound th fraction unfolded 0.7 0 0.1 0.2 0.3 0.4 0.5 0.6...
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
1. Which graph(s) can be used to estimate a Km for an
enzymatic reaction? select all that apply
2. Which graph(s) can be used to calculate an association
constant for a protein binding its ligand? select all that
apply
3. Which graph(s) can be used to estimate protein stability
and to understand folding thermodynamics? select all that
apply
4. Which graph(s) can be used to estimate the equilibrium
constant for the dissociation of a protein-ligand complex? select
all that apply...
1- What is a standard curve and how is it used in biochemistry? 2- Explain how the Lambert-Beer equation is applied in this experiment. 3- Why is a spectrophotometer used to obtain data? 4- Based on the experimental data provided, estimate the amount of amino acid in the given unknown solution by Ninhydrin method. SI No. Amount of amino acid [pg) OD at 570nm 1 2 3 4 5 6 Volume of standard amino acid solution (ml) Blank 0.2 0.4...
1. Which graphs can be used to estimate a Km for an enzymatic
reaction? select all that apply
2. Which graphs can be used to calculate an association
constant for a protein binding its ligand? select all that
apply
3. Which graphs can be used to estimate protein stability and
to understand folding thermodynamics? select all that apply
4. Which graphs can be used to estimate the equilibrium
constant for the dissociation of a protein-ligand complex? select
all that apply...
Please answer what’s required from the following question
correctly.
The curve in the following diagram represents a traverse across the center of a roughly equidi- mensional ore body. The anomaly due to the ore body is obscured by a strong regional anoma- ly. Remove the regional anomaly and then evaluate the anomaly due to the ore body. which has a density contrast of 0.75 g/cm. (Graph values are supplied in the table following the graph.) 0.00 -0.25 0.50 -0.75 0.0...
The following are quality control data for a manufacturing process at Kensport Chemical Company. The data show the temperature in degrees centigrade at five points in time during a manufacturing cycle. Sample x R 1 95.72 1.0 2 95.24 0.9 3 95.18 0.9 4 95.48 0.4 5 95.46 0.5 6 95.32 1.1 7 95.40 1.0 8 95.44 0.3 9 95.08 0.2 9 10 10 95.50 0.6 11 95.80 0.6 12 95.22 0.2 13 95.60 1.3 14 95.22 0.5 15 95.04...
A saturated liquid feed with a composition of 45 mole-% benzene and 55 mole-% toluene is distilled in a plate column at the rate of 250 kmol/h. Overhead product contains 97 mole-% benzene and the bottom product contains 5 mole-% benzene. A total condenser and a partial reboiler are used. The reflux ratio employed is 1.5 times the minimum, RD 1.5 RDmin Find the number of theoretical plates required for this separation, the heat duties of the condenser and reboiler....
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