1) Graphs D and E can be used to calculate the Km value because both these graphs provide with the information of the velocity and the substrate concentration and Km can be easily calculated using the equation:
V = (Vmax [S]) ÷ (KM + [S}) where, V= initial velocity,{S} substrate concentration
2)The association constant can be calculated by considering a graph which encodes details of the bound ligand.So graph A or C may be used.
3)The stability and folding thermodynamics may be understood by considering graphs B or F as stability depends on the effeciency in folding.
4) Equilibrium is also a parameter that determines stability so graph B or F can be used and dissociation of a protein ligand complex can be calculated by A or C
5) The rate can be calculated by a graph that has parameters of velocity of enzyme action therefore D or E may be taken
1. Which graph(s) can be used to estimate a Km for an enzymatic reaction? select all...
1. Which graphs can be used to estimate a Km for an enzymatic reaction? select all that apply 2. Which graphs can be used to calculate an association constant for a protein binding its ligand? select all that apply 3. Which graphs can be used to estimate protein stability and to understand folding thermodynamics? select all that apply 4. Which graphs can be used to estimate the equilibrium constant for the dissociation of a protein-ligand complex? select all that apply...
6. Which graphs can be used to determine the amount of chemical denaturant needed to unfold half of the protein molecules in a sample? select all apply 7. Which graphs can be used to determine whether or not binding a ligand influences binding of additional ligands? select all that apply 8. Which graphs can be used to hypothesize a mechanism of reversible inhibition? select all tgat apply fraction bound th fraction unfolded 0.7 0 0.1 0.2 0.3 0.4 0.5 0.6...
Please help! 4. (3 points) Which set of binding data is likely to represent cooperative ligand binding to an oligomeric protein? Explain! (Note: Yor Theta is the percent saturation of receptor with ligand) a. | [Ligand] (mM) | Y 0.1 0.2 0.4 0.7 0.3 0.5 0.7 0.9 b. 0.3 0.4 0.6 0.3 0.6 0.8
Use the graph to estimate K. 1.0 0.75 Yo₂ 0.5 0.25 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 [L] (M)
LLI (nM) 92 The data at right describe the binding of a ligand to a protein: 0.1 0.07 s [L] is the concentration of free ligand. e is the fraction of sites on the protein that are occupied by the ligand. (Note that some textbooks use terms like v or Y, instead of 6, to denote fractional saturation) 0.4 0.23 0.36 Answer parts (a) and (b) below. 0.55 1.2 (a) Which of the following graphs could be used to estimate...
the following figure showed the velocity graph of blood in the aorta. Estimate the bloods acceleration during all phases of the motion. NECONCIU 2.10 The following figure showed the velocity graph of blood in the aorta. Estimate the blood's (m/s) 1.0- 0.8- 0.6- 0.4- 0.2- 0.0+ -1(5) 0.0 0.1 0.2 0.3 0.4 acceleration during all phases of the motion.
At pharmaceutical companies the goal of many drug discovery experiments is to find improved drugs over existing drugs that bind to a target protein of interest. The graph below shows how "Drug A" binds to a target protein 1. Protein-Ligand Binding Curve for "Drug A" 1 0.9 0.8 0.7 0.6 Y 0.5 0.4 0.3 0.2 0.1 0 0 10 15 20 25 30 35 40 From the graph, estimate the Kd of "Drug A" for the target protein. Report the...
Questions 1. Consider the calibration graph and the absorption spectrum for solutions of metal ion, M * Absorption Spectrum for M Calibration Curve for M 0.8 0.7 1.2 0.6 1 0.5 S 0.8 0.4 0.6 0.3 0.4 0.2 0.1 0.2 0- 0 0.5 0.4 0.3 0.2 0.1 400 450 500 550 600 650 700 [M*1 (Molar) (入) nm a) At what wavelength was the calibration curve obtained? Explain your answer. b) Use the calibration curve to calculate the concentration (M)...
3. Make a plot of a vs. sin6 fon linear graph and draw a best fit line through the data. Extend the best fit line so that it intersects the point at sin@ = 1 . #1 Incline Angle 0-2° (m/s) 0.22 Time Interval (s) Displaceent (m) Average Velocity Average Acceleration 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 m/s/s 0.021 0.022 0.023 0.024 0.025 0.027 0.029 0.030 0.032 0.033 0.1 0.24 0.25 0.27 0.29 0.30 0.32 0.33...
Use contour integration to evaluate the integral 2T 1 I (5 4 cos (0))2 S 0 diagram showing the contour and the singularities Draw and label a For your information, the graph of the integrand is given below. L0- 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1. 7T 2n 4. - Use contour integration to evaluate the integral 2T 1 I (5 4 cos (0))2 S 0 diagram showing the contour and the singularities Draw and label a For...