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define Flow Cytometry edit this to make it clear: detects and measures the physical and chemical...

define Flow Cytometry

edit this to make it clear:

detects and measures the physical and chemical characteristics of a population of cells

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Flow cytometry: It is the technique for cell counting and measurement of different properties of the cell.

It is the laser based technology that measures and analysis different physical and chemical properties of the cells/particles flowing in a stream of fluid through a beam of light

Principle of working of flow cytometer: counter principle+principles of laminar flow+electrostatics+optics and light scattering= flow cytometry

Components:fluidics, optics, and electronics

Working:

  1. Biological sample will be labeled with a fluorescent marker
  2. Cells move in a linear stream through a focused light source (laser beam)
  3. Fluorescent molecule gets activates and emits light that is filtered and detected by sensitive light detectors(usually a photomultiplier tube)
  4. Conversion of analog fluorescent signals to digital signal

Clinical application: Immunophenotyping, functional assay, cell counting, cell sorting.

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