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You have an unknown bacterium. You decide to plate it on an MSA blate. After 24 hours the plate turns from red to yellow. Thi
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Answer #1

D.Your bacteria can ferment mannitol

MSA-Mannitol Salt Agar medium contains the sugar mannitol and the pH indicator phenol red.If it is turned yellow from red which means organism present in this medium can ferment mannitol,by this an acidic by product will be formed that is actually causing phenol red to yellow in agar.Pathogenic organisms under staphylococci will ferment mannitol. The bacteria can grow this plate should be halophilic(Salt loving) because of this medium contains high concentration of salt.

other options are incorrect related to given observations

In this experiment fermentation of mannitol is occurring not glucose to lactose or galactose,gram negative bacteria will not grow in MSA test,because of the high concentration of salt in the medium.

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