Question

Describe the steps you would take to isolate, cultivate, and identify a microbial pathogen from a urine sample. Remember and take into account the procedures routinely employed in microbiology.

Answer 1

Answer: there are following steps for the urime Culture:- o place the urime Sample on Agar plats which are the mutrients Media for the growth of the pathogen. After this micubate the plats at Body Hmperature. Amy pathogen which is present in the urime sample will grow unto the Colomies im mext 24 to 48 hours from the Colomies we con identify which pathogen is present in the urime Sample the above method is good for the single type of pathogen. It may be a chance that there are more than one pathogen present in the urime sample. this is due to contamination during the urime Collection. After the identification the grow exconimes the microorganisms ander the Microscope. Different pathogus varies in the colors and shapes. For example, Escherichia coli will appear pink under Microscope Lactobacillus will appear as thin purple rods. For some of the pathoques, do not Require any investigation. But for some pathogens further investigation is meeded. If there is mo or little growth After 24 to 48 hours of Incubation, the arime Culture is considered megative for pathogens staining is done to the mi purthur investic