Explain how we can visualize cAMP in cells using FRET (2 pts) Please no cursive handwriting,...
Explain how we can visualize cAMP in cells using FRET (2 pts)
Please no cursive handwriting, I can't read cursive.
Homework Answers
The FRET-based cAMP indicator includes two major components :
- a cAMP sensor, and
- a chromophore donor and acceptor fused to the cAMP sensor.
Changes in conformation, or distance between the interacting protein partners, influence the distance or orientation of the two fluorophores and thus the energy transfer efficiency. The real-time cAMP imaging is based on fluorescein-labeled PKA-C and rhodamine-labeled PKA-R subunits, which have been microinjected into cells. Within a resting cell, FRET occurs between the two fluorophores under low cAMP conditions, while an increase within cAMP concentration is associated with a decrease in FRET due to dissociation of PKA subunits.
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Explain how we can visualize cAMP in cells using FRET (2
pts)
Please no cursive handwriting,...
Please do not write in cursive, as I cannot read cursive. Please explain how you got...
Please do not write in cursive, as I cannot read cursive. Please
explain how you got the answers and show the work. Thank you very
much
in-too An does not? 1) Could there be a sequence {an}= {f (n)} such that limita—700 f (x) exists, but limit 2) Could limit noo An exists but not for limitx-700 f(x) 3) Discuss your options , you can give an example to enhance your reasoning.
write about: how we can control the cells' Oxygen in Nanoparticle Sensor Technology please not handwriting
write about: how we can control the cells' Oxygen in Nanoparticle Sensor Technology please not handwriting
In signaling through the β-adrenergic receptor the same hormone can have different effects on difference cells....
In signaling through the β-adrenergic receptor the same hormone can have different effects on difference cells. Explain what happens to each of the following and what last signaling step is common to all (2 pts each): A. Heart B. Skeletal Muscle C. Smooth muscle Please no cursive handwritting, I cant read cursive.
Explain in terms of channel opening/closing/inactivation and ion movements what is going on at the indicated...
Explain in terms of channel opening/closing/inactivation and ion movements what is going on at the indicated points of the action potential. (2 points each) A. Threshold B. Rising phase C. Declining phase D. Undershoot E. Return to baseline Please no cursive handwriting, I can't read cursive.
Please explain the whole problem and make sure I can read the handwriting please. I will...
Please explain the whole problem and make sure I can read the
handwriting please. I will rate accordingly. Thanks in
advanced.
2. (a) Use the Gram-Schmidt orthonormalization process to transform B = {(1, -2, 2), (2, 2, 1), (2, -1, -2)} into an orthonormal basis for R3. (b) What are the coordinates of (1, 2, 3) relative to the orthonormal basis you found in part (a)?
please write 2 sentences shot answer In the comparative advantage model, how do we visualize the...
please write 2 sentences shot answer
In the comparative advantage model, how do we visualize the gains from trade?
PLEASE I NEED CLEAR HANDWRITING 2. (16 pts) Technology Readiness Level a) (4 pts) What does...
PLEASE I NEED CLEAR
HANDWRITING
2. (16 pts) Technology Readiness Level a) (4 pts) What does “Technology Maturation Point / Technology Readiness Level" mean? Please explain shortly. b) (4 pts) If 4 years of time is needed to complete the steps of product development, how many years are needed to complete the "Technology Development" studies? c) (8 pts) Please write down the four main stages of “Technology Development" according to NASA Technology Readiness Level Scale.
Please no cursive and explain answer so I can understand. Exercise A Using the average cost...
Please no cursive and explain answer so I can understand.
Exercise A Using the average cost method, compute the equivalent units of production in each of the following cases: 1. Units started in production during the month, 72,000; units completed and transferred, 52,800 and units in process at the end of the month (100% complete as to materials; 60% complete as to conversion), 19,200. (There was no beginning inventory.) 2. Units in process at the beginning of the month (100%...
I need a flow chart for this, please do not write in cursive. so i can...
I need a flow chart for this, please do not write in cursive. so i can read it please Algorithm for the Program: 1.Start reading the character c till the while loop ends. 2.Write the condition if the given is not character,Stops the program. 3.Call the function ischar() to determine if letter is character or not. 4.In the function check smaller case alphabet and return ASCII index of c-'a'+1. 5.If not,check bigger case alphabet and return ASCII index of c-'A'+1....
Please help with the 2 questions. For 6, I think we can use GFP, but I'm...
Please help with the 2 questions.
For 6, I think we can use GFP, but I'm not sure if it's right
and I don't know how to describe the process.
5. If you are given a monoclonal antibody and a polyclonal antibody against a given protein, which do you think is more likely to have blocking activity (to inhibit the function of the given protein)? 6. The Zebra fish is a model organism used to study vertebrate development. One feature...
Please do not write in cursive, as I cannot read cursive. Please
explain how you got the answers and show the work. Thank you very
much
in-too An does not? 1) Could there be a sequence {an}= {f (n)} such that limita—700 f (x) exists, but limit 2) Could limit noo An exists but not for limitx-700 f(x) 3) Discuss your options , you can give an example to enhance your reasoning.
Please explain the whole problem and make sure I can read the
handwriting please. I will rate accordingly. Thanks in
advanced.
2. (a) Use the Gram-Schmidt orthonormalization process to transform B = {(1, -2, 2), (2, 2, 1), (2, -1, -2)} into an orthonormal basis for R3. (b) What are the coordinates of (1, 2, 3) relative to the orthonormal basis you found in part (a)?
please write 2 sentences shot answer
In the comparative advantage model, how do we visualize the gains from trade?
PLEASE I NEED CLEAR
HANDWRITING
2. (16 pts) Technology Readiness Level a) (4 pts) What does “Technology Maturation Point / Technology Readiness Level" mean? Please explain shortly. b) (4 pts) If 4 years of time is needed to complete the steps of product development, how many years are needed to complete the "Technology Development" studies? c) (8 pts) Please write down the four main stages of “Technology Development" according to NASA Technology Readiness Level Scale.
Please no cursive and explain answer so I can understand.
Exercise A Using the average cost method, compute the equivalent units of production in each of the following cases: 1. Units started in production during the month, 72,000; units completed and transferred, 52,800 and units in process at the end of the month (100% complete as to materials; 60% complete as to conversion), 19,200. (There was no beginning inventory.) 2. Units in process at the beginning of the month (100%...
Please help with the 2 questions.
For 6, I think we can use GFP, but I'm not sure if it's right
and I don't know how to describe the process.
5. If you are given a monoclonal antibody and a polyclonal antibody against a given protein, which do you think is more likely to have blocking activity (to inhibit the function of the given protein)? 6. The Zebra fish is a model organism used to study vertebrate development. One feature...
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