What is the concentration of bacteria in the sample below?
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What is the concentration of bacteria in the sample below?
What is the concentration of bacteria...
1)How many microliters of original sample are required to produce a final dilution of 10-2 in...
1)How many microliters of original sample are required to produce a final dilution of 10-2 in a total volume of 5 mL? 1 microliter is 10-6 L or 10-3 mL. 2)A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 197 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture?...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates,...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
I'm not sure if I completed question 3 correctly. Also, I need help determing question 4....
I'm
not sure if I completed question 3 correctly. Also, I need help
determing question 4.
Y-0.301 6.51-hardts.al 3. A solution containing 100 mL of bacterial broth is subcultured onto 5 plates using the pour-plate method. Each plate receives broth according to the following Table. The Table also lists the results colony counts after 24 hours of incubation. Calculate the number of bacteria per milliliter of the bacterial broth for each of the dilutions. Plate Volume Broth Colonies 331 2...
microbiology 1. Use the following example of a series of dilutions and plating to answer the...
microbiology
1. Use the following example of a series of dilutions and plating to answer the problems below. 1 ml 1 ml 1 ml 1 mL 1 mL 9 mL 9 mL 9 ml 9 ml Sample of E.coli 1:10 1:100 1:1000 1:10,000 1:100,000 10- 10- 60 1 mL 1 mL Tim Time O O O O O Colony counts: Colony counts: Toon Too many to count 1354 1354 290 290 75 75 10 10 A. What is the original...
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1....
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you...
Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would add to your sample to accomplish that? A. 10 ml B. 9 ml C. 9.9 ml D. 0.9 ml E. 0.99 ml 2. You have 0.5 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would...
Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you...
Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would add to your sample to accomplish that? A. 10 ml B. 9 ml C. 9.9 ml D. 0.9 ml E. 0.99 ml 2. You have 0.5 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would...
Please help me solve these problems. I don't understand 1. Placing a 0.5 ml sample of...
Please help me solve these
problems. I don't understand
1. Placing a 0.5 ml sample of bacteria into a 4.5 ml sterile water, then transferring a 0.5 ml sample from that dilution to another 4.5 ml sterile water produce a dilution. 2. A technologist in a hospital lab who is trying to determine the concentration of Staphylococcus aureus in a urine sample completed a total plate count. After plating 0.1mL in duplicate, and incubating the plates at 37°C for 24...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
I'm
not sure if I completed question 3 correctly. Also, I need help
determing question 4.
Y-0.301 6.51-hardts.al 3. A solution containing 100 mL of bacterial broth is subcultured onto 5 plates using the pour-plate method. Each plate receives broth according to the following Table. The Table also lists the results colony counts after 24 hours of incubation. Calculate the number of bacteria per milliliter of the bacterial broth for each of the dilutions. Plate Volume Broth Colonies 331 2...
microbiology
1. Use the following example of a series of dilutions and plating to answer the problems below. 1 ml 1 ml 1 ml 1 mL 1 mL 9 mL 9 mL 9 ml 9 ml Sample of E.coli 1:10 1:100 1:1000 1:10,000 1:100,000 10- 10- 60 1 mL 1 mL Tim Time O O O O O Colony counts: Colony counts: Toon Too many to count 1354 1354 290 290 75 75 10 10 A. What is the original...
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would add to your sample to accomplish that? A. 10 ml B. 9 ml C. 9.9 ml D. 0.9 ml E. 0.99 ml 2. You have 0.5 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would...
Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would add to your sample to accomplish that? A. 10 ml B. 9 ml C. 9.9 ml D. 0.9 ml E. 0.99 ml 2. You have 0.5 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would...
Please help me solve these
problems. I don't understand
1. Placing a 0.5 ml sample of bacteria into a 4.5 ml sterile water, then transferring a 0.5 ml sample from that dilution to another 4.5 ml sterile water produce a dilution. 2. A technologist in a hospital lab who is trying to determine the concentration of Staphylococcus aureus in a urine sample completed a total plate count. After plating 0.1mL in duplicate, and incubating the plates at 37°C for 24...
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