Homework Answers
Answer : Sample C (N14 N14 and N14 N15)
Start with medium containing N15 and the DNA replication results in both strands containing N15.
Then, N14 containing medium is supplied and after one generation, all the DNA contain one strand with N14 and the other strand with N15.
In the second generation with same N14 medium, half of the DNA will have two strands with N14 and another half of the DNA will have one strand with N15 and other strand with N14.
DNA replication is semiconservative. Therefore, proportion of N15 decreases gradually.
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QUESTION 3 What were the actual results of the Meselson and Stahl experiment after two generations?...
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If DNA was replicated via a conservative mechanism and you repeated the Meselson and Stahl experiment, what would you expect to see when the bacteria was allowed to replicate 3 times (60 min) in the N14 medium and DNA was extracted and centrifuged? Select one: a. several bands (uncountable) forming a smear across the tube b. Two bands one formed using only N14 and one formed by N15 c. it is impossible to determine given the information provided d. Two...
QUESTION 27 The Meselson and Stahl experiment was used to compare the conservative, semiconservative and dispersive models of DNA replication. They cultured E. coli cells for several generations in a medium containing heavy nitrogen, 15 N. They then transferred the bacteria to a medium containing a lighter nitrogen isotope, 14 N. Samples were taken after the first round of DNA replication; another sample was taken after the second round of replication. They extracted DNA and centrifuged each sample. Explain (in...
5. (4 points) Imagine that Meselson and Stahl performed their experiment on a sample of 8 cells, each containing one copy of double-stranded DNA genome. These 8 cells had been growing on normal 14N medium. You then grew the cells for 3 generations in medium containing 1SN. How many cells would there be with DNA of 14N, 14N and 15N, and 15N composition, respectively?
Question 45 Which of the following body axis is determined by the sperm entry point in the developing frog embryo? Not yet answered Points out of 2.50 Flag question Select one: O a. none of the body axis are determined by the site of sperm entry O b. Anterior - posterior O c. right-left O d. Dorsal - ventral Question 46 Not yet answered If DNA was replicated via a conservative mechanism and you repeated the Meselson and Stahl experiment,...
After Watson and Crick proposed the double helix model of DNA, three models for DNA replication were proposed: conservative, semiconservative and dispersive. The following figures illustrate the Meselson and Stahl experiment, which demonstrated the correctness of the semiconservative model of DNA replication N15 N14 How would the results be if the correct model was the conservative or dispersive: 1. Conservative: 20 40 60 80 Time (min.) 2. Dispersive 20 40 60 80 Time (min.)
In a famous experiment, Matthew Meselson and Franklin Stahl grew E.coli cells in a medium containing only the "her isotope of nitrogen, SN. These cells were then transferred to a medium with the "light" isotope of nitrogen, "N. The results of the experiment supported the hypothesis of semiconservative replication, which was proposed by Watson and Crick. Heavy DNA ('N DNA), hybrid DNA, and light DNA (N DNA) can be separated by centrifugation. If cells containing 1SN DNA are transferred to...
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