what are some of the uses for purified DNA in a modern lab?
Homework Answers
Uses for purified DNA in a modern lab -a) .It can be used for transfection in the cell culture for protein expression to characterize the cellular function.b). It also can be used as a template DNA to amplify the particular domain of gene by the help of polymerase chain reaction.c) Can be used to diagnose the pathogenic condition.d) can be used in bacterial transformation to study the protein expression and in interaction studies with other proteins(immunoprecipitations).
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What is Modern Physics? Discuss some of the discoveries that led to the development of Modern physics. What is special relativity? What is general relativity? Discuss the postulates of relativity. What is the consistent law principle? According to relativity describe how a falling object looks to an observer in a windowless bus.
Why are we treating our purified PCR products with a DNA polymerase before adding them to...
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If you placed some purified C5a at the base of a blister, what effect would you...
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You've identified and purified a novel closed circular viral DNA, and want to treat it with a typ...
You've identified and purified a novel closed circular viral DNA, and want to treat it with a type II restriction enzyme used in BCH370. This DNA has a single restriction site contained within the following sequence: 5' GGGTTATGAATTCTACA 3' 3, CCCAATACTTAAGATGT 5, a) Indicate the probable region identified by the restriction enzyme, and explain how you can identify the recognition sequence based on certain criteria. (2 marks) b) You then attempt to identify whether or not the viral vector contains...
What are some possible alternatives to the current modern agriculture system(s)?
What are some possible alternatives to the current modern agriculture system(s)?
1- Which are the differences between the PCR we used in the lab and DNA replication...
1- Which are the differences between the PCR we used in the lab and DNA replication in human cells? A. PCR replicates specific DNA segments while in human cells all the DNA is replicated B. PCR uses high temperature to dissociate the DNA strands while DNA replication in human cells uses helicase C. PCR uses a high temperature resistant polymerase while the DNA polymerase from human cells would denature at high temperatures D. A and B are true E. A,...
You have prepared some plasmid DNA in lab and need to know its concentration so you...
You have prepared some plasmid DNA in lab and need to know its concentration so you can load 1 ug on a gel. You dilute 1 uL with 99 uL of water and the resulting sample has an absorbance of 0.075. What volume of the original sample do you need to load onto the gel? (Show math for partial credit and make sure you are answering the question I am actually asking.)
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1. In Lab 1, your goal will be to isolate a purified sample of neutral naproxen....
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What is the difference between Modern Medicine like chemotherapy and Natural Medicine like herbs ? What...
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You've identified and purified a novel closed circular viral DNA, and want to treat it with a type II restriction enzyme used in BCH370. This DNA has a single restriction site contained within the following sequence: 5' GGGTTATGAATTCTACA 3' 3, CCCAATACTTAAGATGT 5, a) Indicate the probable region identified by the restriction enzyme, and explain how you can identify the recognition sequence based on certain criteria. (2 marks) b) You then attempt to identify whether or not the viral vector contains...
1. In Lab 1, your goal will be to isolate a purified sample of neutral naproxen. Recall from lecture that (S)-naproxen is an effective pain killer, while (R)- naproxen is a liver toxin. Draw the safe (S) stereoisomer. What is the relationship between the two stereoisomers? 2. You will begin Lab I with a tablet of sodium naproxen. The molecular weight of neutral naproxen is -230 g/mol. Calculate the molecular weight of sodium naproxen, to the nearest g/mol. 3. Based...
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