what are some of the uses for purified DNA in a modern lab?
Homework Answers
Uses for purified DNA in a modern lab -a) .It can be used for transfection in the cell culture for protein expression to characterize the cellular function.b). It also can be used as a template DNA to amplify the particular domain of gene by the help of polymerase chain reaction.c) Can be used to diagnose the pathogenic condition.d) can be used in bacterial transformation to study the protein expression and in interaction studies with other proteins(immunoprecipitations).
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If you placed some purified C5a at the base of a blister, what effect would you...
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You've identified and purified a novel closed circular viral DNA, and want to treat it with a typ...
You've identified and purified a novel closed circular viral DNA, and want to treat it with a type II restriction enzyme used in BCH370. This DNA has a single restriction site contained within the following sequence: 5' GGGTTATGAATTCTACA 3' 3, CCCAATACTTAAGATGT 5, a) Indicate the probable region identified by the restriction enzyme, and explain how you can identify the recognition sequence based on certain criteria. (2 marks) b) You then attempt to identify whether or not the viral vector contains...
What are some possible alternatives to the current modern agriculture system(s)?
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1- Which are the differences between the PCR we used in the lab and DNA replication...
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You've identified and purified a novel closed circular viral DNA, and want to treat it with a type II restriction enzyme used in BCH370. This DNA has a single restriction site contained within the following sequence: 5' GGGTTATGAATTCTACA 3' 3, CCCAATACTTAAGATGT 5, a) Indicate the probable region identified by the restriction enzyme, and explain how you can identify the recognition sequence based on certain criteria. (2 marks) b) You then attempt to identify whether or not the viral vector contains...
1. In Lab 1, your goal will be to isolate a purified sample of neutral naproxen. Recall from lecture that (S)-naproxen is an effective pain killer, while (R)- naproxen is a liver toxin. Draw the safe (S) stereoisomer. What is the relationship between the two stereoisomers? 2. You will begin Lab I with a tablet of sodium naproxen. The molecular weight of neutral naproxen is -230 g/mol. Calculate the molecular weight of sodium naproxen, to the nearest g/mol. 3. Based...
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