Question

10 E. O B 6 E- 4 BII -A complete digest with EcoRI + BamHI of 12?g of the above fragment was performed. Indicate the amount which would be obtained in ?g of the 4 Kbp fragment.

0 0
Add a comment Improve this question Transcribed image text
Answer #1

Answer :-

1 μg 1 Kbp
4μg 2Kbp
3μg 3Kbp
4μg 4Kbp

the fragment between the BamH1 and ECoR1 is the 4Kbp fragment obtained after restriction digestion of the given 12μg DNA,

The above table predicts you the pattern of the restriction digestion obtained lengths and amount respectively,

4 Kbp fragment obtain 4μg DNA fragment.

Add a comment
Know the answer?
Add Answer to:
A complete digest with EcoRI + BamHI of 12?g of the above fragment was performed. Indicate...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
  • How many times do the enzymes BamHI and EcoRI cut the S. cerevisiae genome within the...

    How many times do the enzymes BamHI and EcoRI cut the S. cerevisiae genome within the region spanned by the probe? I am very confused and don't understand this question? Can someone plz EXPLAIN it to me A Southern hybridization was performed on genomic digests of Saccharomyces cerevisiae. Following migration on an agarose gel, the digests were probed with a 3Kbp radioactive probe. The results obtained following the hybridization are presented below Size of hybrids (Kbp Enzyme BamHI EcoRI EcoRI+...

  • - BamHI Ecort 4000 400 EcoRI 5kb 100 2000 I Target region of 770 bp in...

    - BamHI Ecort 4000 400 EcoRI 5kb 100 2000 I Target region of 770 bp in length has been amplified Plan to digest the DNA amplican with restrictich enzyme Egel, and done the resulting longest fragment into Eael site of 5 KB plasmid. 4. You now run the four RE digests of the recombinant plasmid from question (4) on an agarose gel and stain it with ethidium bromide (EtBr). Indicate your expected profile on the gel below. Clearly indicate the...

  • 9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA...

    9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA with the enzymes BamHI and HindIII separately and then load the fragments into an agarose gel and perform electrophoresis. Next, you perform a Southern analysis using the 4,878-bp EcoRI lambda fragment as a probe. a. Draw a picture of the electrophoresis gel, using the outline of the stained electrophoresis gel in Worksheet 16.IIIB (the two smallest HindIII fragments will run off the gel.) b....

  • Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....

    Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...

  • If the target DNA (the 3.266 Kb E. coli genomic Bam HI fragment) has the same restriction sites on each end, there are t...

    If the target DNA (the 3.266 Kb E. coli genomic Bam HI fragment) has the same restriction sites on each end, there are two possible orientations for the target DNA to insert into the plasmid. The following restriction enzymes would cut the 3.266 kb Bam HI genomic fragment containing the RecA gene once or twice or not at all. In the tables below, list the expected DNA fragment sizes for the two possible orientations. Round the DNA fragment sizes to...

  • See image please show full solution, (Indicate your answers to two digits after the decimal) 8....

    See image please show full solution, (Indicate your answers to two digits after the decimal) 8. You have two plasmid preparations "A and "B" (illustrated below) each at concentrations of 100nguL. You wish to subclone a 1.5 Kbp EcoRI fragment from plasmid·A" into the unique EcoRI site of a 3.0Kbp vector"B". EcoRI 1.5 Kbp Total size 3.0Kbp BT Total size 7.5Kbp Complete the following table to indicate the volumes of each ingredient required for the preparation of a 50μL ligation...

  • please help if you can 3. You cloned a 20 kb piece of DNA, which contains...

    please help if you can 3. You cloned a 20 kb piece of DNA, which contains restriction sites as shown below. 281534 5 B 4 & 5 B = BamHl site, E = EcoRI site, H = Hindill site Numbers above the segments represent the sizes of the regions in kb. Draw and label (in the agarose gel below) the sizes of the fragments you would expect to see after complete digestion of this piece of DNA with the following...

  • You are preparing to clone a DNA fragment into a plasmid vector. You start by linearizing...

    You are preparing to clone a DNA fragment into a plasmid vector. You start by linearizing your plasmid (concentration = 500 ng/μl) with EcoRI, which is provided in a standard 50% glycerol solution at 10 units/μl. Your enzyme also comes with an appropriate 10X reaction buffer.  Taking into account the final allowable glycerol concentration, you want to add the maximum amount of EcoRI, to achieve complete digestion of 1 μg plasmid DNA in a total volume of 20 μl. Fill...

  • QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated...

    QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...

  • QUESTION 1 G E. H. C A Finish Start D. Complete the network activity diagram using the table below to answer questio...

    QUESTION 1 G E. H. C A Finish Start D. Complete the network activity diagram using the table below to answer questions 1-15. The expected activity completion times are provided in days. Each question is 1 point. Most Probable Activity Optimistic Pessimistic A 7 B 12 C 5 12 7 E 10 15 20 6 9 18 G 6 13 H 11 What is the early start (ES) time for activity F? 13 12 10 None of the above are...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT