Homework Answers
DNA polymerase requires a free 3' OH group for initiation of synthesis of the daughter strand. DNA polymerase can synthesise in only one direction by extending the 3' end of the existing nucleotide chain. Hence DNA polymerase moves along the template strand in a 3' to 5' direction, and the daughter strand is formed in a 5'-3' Direction.
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why does dna polymerase only tun 3' to 5' direction?
ON) Why does DNA polymerase only...
Why does a new DNA strand elongate only in the 5' to 3' direction during DNA...
Why does a new DNA strand elongate only in the 5' to 3' direction during DNA replication? The polarity of the DNA molecule prevents addition of nucleotides at the 3' en Replication must progress toward the replication fork. DNA polymerase can add nucleotides only to the free 3' end. DNA polymerase begins adding nucleotides at the 5' end of the template,
QUESTION 13 A new DNA strand elongates only in the to direction because A) DNA polymerase...
QUESTION 13 A new DNA strand elongates only in the to direction because A) DNA polymerase begins adding nucleotides at the 5' end of the template B) the polarity of the DNA molecule prevents addition of nucleotides at the 3'end OB) the C) replication must progress toward the replication fork D) DNA polymerase can add nucleotides only to the free 3'end QUESTION 14
why is it that the DNA polymerase needs 4 deoxyribonucletides else RNA polymerase need only 2NTP
why is it that the DNA polymerase needs 4 deoxyribonucletides else RNA polymerase need only 2NTP
4. The enzyme DNA polymerase has two important activities: 5-10-3 polymerase and 3 '-10-5'exonuclease. The 5'-to-3'...
4. The enzyme DNA polymerase has two important activities: 5-10-3 polymerase and 3 '-10-5'exonuclease. The 5'-to-3' polymerase makes one misincorporation per 100,000 incorporations. A misincorporation occurs when the "wrong" nucleotide-residue is added to the growing DNA-molecule, such that the added residue is NOT complementary in the normal Watson-Crick way (G with C, A with T) to the residue in the template DNA. Normally, the 3'-to-5'exonuclease corrects most of these misincorporations, reducing the misincorporation rate to one in 10,000,000. If a...
Describe how the carboxy terminal domain (CTD) of RNA polymerase II is involved in initiation and...
Describe how the carboxy terminal domain (CTD) of RNA polymerase II is involved in initiation and elongation of transcription. HTML Editori B I VA - A - Ix E 1 1 1 x X, DE T TH 12pt Paragraph Tube O words
Name the known DNA polymerases. Which DNA polymerases replicate DNA in the 5’-3’ direction? Which DNA...
Name the known DNA polymerases. Which DNA polymerases replicate DNA in the 5’-3’ direction? Which DNA polymerase is responsible for elongating the replicating strand? Which DNA polymerases function in DNA repair? Which DNA polymerase can remove either DNA or RNA nucleotides from the 5’ end of a nucleic acid strand?
You created a mutant form of DNA polymerase in which the 3'to 5' exonuclease (proofreading] function...
You created a mutant form of DNA polymerase in which the 3'to 5' exonuclease (proofreading] function has been destroyed, although its polymerase activity is still fine. Which of the following properties do you expect the mutant polymerase to have? It will polymerize in both the 5-to-3' direction and the 3'-to-5' direction. o It will likely make a new strand of DNA (same length) slower than a normal polymerase. It will be more likely to generate thymidine dimers. As it gets...
Explain the 5’-to-3’ directional activity of DNA polymerase. Please give an explanation for the inability of...
Explain the 5’-to-3’ directional activity of DNA polymerase. Please give an explanation for the inability of this enzyme to synthesize DNA in opposite direction (3’-to-5’).
Question 34 12 pts Indicate whether the following statements are true for DNA replication only (type...
Question 34 12 pts Indicate whether the following statements are true for DNA replication only (type DNA next to the statement), RNA transcription only (type RNA next to the statement); both replication and transcription (type BOTH next to the statement); or neither replication or transcription (type NONE next to the statement). (10 points, 2 points each) A. The new strand is synthesized in the 3' to 5' direction. B. A primer is required. C. A template is required. D. Polymerases...
SELLUN 9. Contrast the functions of DNA polymerase III and DNA polymerase I. 10. Why do...
SELLUN 9. Contrast the functions of DNA polymerase III and DNA polymerase I. 10. Why do are the start points of DNA replication in eukaryotic and prokaryotic organisms AT rich? 11. What is the start point of DNA replication called in E. coli? What is it called in humans? 12. In DNA replication in E. coli which enters the replication fork first, DnaA protein, or Helicase? 13. In eukaryotes, two proteins are involved in linking DNA replication to S phase...
Why does a new DNA strand elongate only in the 5' to 3' direction during DNA replication? The polarity of the DNA molecule prevents addition of nucleotides at the 3' en Replication must progress toward the replication fork. DNA polymerase can add nucleotides only to the free 3' end. DNA polymerase begins adding nucleotides at the 5' end of the template,
QUESTION 13 A new DNA strand elongates only in the to direction because A) DNA polymerase begins adding nucleotides at the 5' end of the template B) the polarity of the DNA molecule prevents addition of nucleotides at the 3'end OB) the C) replication must progress toward the replication fork D) DNA polymerase can add nucleotides only to the free 3'end QUESTION 14
4. The enzyme DNA polymerase has two important activities: 5-10-3 polymerase and 3 '-10-5'exonuclease. The 5'-to-3' polymerase makes one misincorporation per 100,000 incorporations. A misincorporation occurs when the "wrong" nucleotide-residue is added to the growing DNA-molecule, such that the added residue is NOT complementary in the normal Watson-Crick way (G with C, A with T) to the residue in the template DNA. Normally, the 3'-to-5'exonuclease corrects most of these misincorporations, reducing the misincorporation rate to one in 10,000,000. If a...
Describe how the carboxy terminal domain (CTD) of RNA polymerase II is involved in initiation and elongation of transcription. HTML Editori B I VA - A - Ix E 1 1 1 x X, DE T TH 12pt Paragraph Tube O words
You created a mutant form of DNA polymerase in which the 3'to 5' exonuclease (proofreading] function has been destroyed, although its polymerase activity is still fine. Which of the following properties do you expect the mutant polymerase to have? It will polymerize in both the 5-to-3' direction and the 3'-to-5' direction. o It will likely make a new strand of DNA (same length) slower than a normal polymerase. It will be more likely to generate thymidine dimers. As it gets...
Question 34 12 pts Indicate whether the following statements are true for DNA replication only (type DNA next to the statement), RNA transcription only (type RNA next to the statement); both replication and transcription (type BOTH next to the statement); or neither replication or transcription (type NONE next to the statement). (10 points, 2 points each) A. The new strand is synthesized in the 3' to 5' direction. B. A primer is required. C. A template is required. D. Polymerases...
SELLUN 9. Contrast the functions of DNA polymerase III and DNA polymerase I. 10. Why do are the start points of DNA replication in eukaryotic and prokaryotic organisms AT rich? 11. What is the start point of DNA replication called in E. coli? What is it called in humans? 12. In DNA replication in E. coli which enters the replication fork first, DnaA protein, or Helicase? 13. In eukaryotes, two proteins are involved in linking DNA replication to S phase...
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