Explain Base hydrolysis of DNA and RNA
- What possible products would you see?
- Which is more readily seen and why
- for PCR why do we add dNTPS?
Explain Base hydrolysis of DNA and RNA - What possible products would you see? - Which...
you want to make RNA in vitro, how would you do that? meaning what would you add to get RNA in PCR instead of DNA
If you see a DNA sequence such as ATGGC, which base makes the 3' end? Which base/bases has a free hydroxyl group? If this was RNA (AUGGC), which base/bases have a free hydroxyl group
Could please explain what would happen in these scenarios 1. If the base sequences of the guide RNA and the target DNA do NOT match If the base sequences of the guide RNA and the target DNA do match
1. Explain the difference between an intron and an exon. 2. Why do the two possible PCR products differ in size by 300 base pairs? 3. Explain how agarose electrophoresis separates DNA fragments. Why does a smaller DNA fragment move faster than a larger one? 4. What kind of controls are run in this experiment? Why are they important? Could others be used?
Briefly describe the structure of RNA. What makes it different than DNA? What do DNA and RNA stand for? What are their roles? What are the DNA base pairing rules? What are the RNA base pairing rules? Describe what the following abbreviations stand for (ACGTU). What is the “norm” for lactose digestion in adults? Why are some people lactose intolerant? Is lactose tolerance relatively recent or ancient?
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
What is the importance of the 2′ hydroxyl group in the ribose sugar of an RNA molecule? Check all that apply. View Available Hint(s) Hint 1. Properties of an hydroxyl groupopened hint A hydroxyl group has the formula OH. Water, alcohols, carboxylic acids, and other hydroxy-containing compounds can be deprotonated owing to the high electronegativity of oxygen, resulting in a stable conjugate base. Hydroxy-containing compounds are also able to form hydrogen bonds, which lead to higher boiling and melting points...
Explain at least one possible reason why you might have the following results in an agarose gel electrophoresis of your PCR products. In addition to your DNA PCR sample, you also ran a Hi-Lo Marker, a positive PCR control, and a negative PCR control. 16. Scenario C: Your Hi-Lo marker showed up but there were no bands in the lanes for your PCR positive control, DNA PCR sample or negative PCR control.
What is the purpose in a PCR reaction for each of the following reagents? Taq Taq buffer dNTPs Forward primer Reverse primer Genomic DNA What do you think would happen if you forgot to add your Reverse primer when you did a PCR?
Draw the base cytosine from RNA and DNA. • You do not have to consider stereochemistry. • You do not have to explicitly draw H atoms. co - 1,000 - ChemDoodle