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If you see a DNA sequence such as ATGGC, which base makes the 3' end? Which...
please help me with 6 and 7 sorry 7 and 8 Pyruvate. Km = 400 micromolar...
please help me with 6 and 7
sorry 7 and 8
Pyruvate. Km = 400 micromolar HCO3 km = 1000 micromolar ATP. Km = 60 micromolar 7 Which protein sequence is mostly likely to undergo phosphorylation in a cell? 0 (2 points) GRTGTK GALNO GWPDE GCRKK 8 If you see a DNA sequence such as ATGGC, which base makes the 3' end? Which base/bases has a free hydroxyl group? If this was RNA (AUGGC), which base/bases have a free hydroxyl...
5) The following sequence of bases is present along one chain of a DNA double-helix that...
5) The following sequence of bases is present along one chain of a DNA double-helix that has opened up at a replication fork, and synthesis of an RNA primer on this template begins by copying the base in bold. 3' - ... TCT GAT ATC AGT ACG ... - 5' a) If the RNA primer consists of 8 nucleotides, what is its base sequence? b) In the intact RNA primer, which nucleotide has a free hydroxyl (-OH) terminus and what...
Review. Consider how one might label the 5'ends of DNA for a footprinting experiment where you...
Review. Consider how one might label the 5'ends of DNA for a footprinting experiment where you visualize where on the DNA a transacting protein binds to the cis acting promoter sequence The 5 phosphate(s) at the S'ends a DNA can be removed using a phosphatase, like calf intestinal phosphatase (CIP), in vitro, leaving a 5-hydroxyl group at the end of DNA CIP has no impact on the phosphates along the backbone of the DNA, just the 5' end phosphate and...
Unwinds DNA strand to make replication fork. Adds free nucleotides to the growing daughter DNA strands...
Unwinds DNA strand to make replication fork. Adds free nucleotides to the growing daughter DNA strands Adds short pieces of RNA to help DNA polymerase start Removes RNA and replaces with DNA Fuses or "glues" fragments of DNA together Proofreads or edits the DNA, checking for mistakes Given the following, DNA Sequence, what is the new daughter strand? (Did you label the 5' and 3' ends?) What is the name of the "fragments" of DNA on the lagging strand after...
Explain Base hydrolysis of DNA and RNA - What possible products would you see? - Which...
Explain Base hydrolysis of DNA and RNA - What possible products would you see? - Which is more readily seen and why - for PCR why do we add dNTPS?
the several other 10.4 to show t 3. The base uracil substitutes for the base thymine in RNA. Complete Table ways...
the several other 10.4 to show t 3. The base uracil substitutes for the base thymine in RNA. Complete Table ways RNA differs from DNA Table 10.4 DNA Structure Compared with RNA Structure RNA Sugar Bases Strands Helix DNA Deoxyribose Adenine, guanine, thymine, cytosine Double stranded with base pairing Yes Complementary Base Pairing Complementary base pairing occurs between DNA and RNA. The RNA base uracil pairs with the DNA base adenine; the other bases pair as shown previously. Complete Table...
48) If one strand of a DNA double helix ha one strand of a DNA double...
48) If one strand of a DNA double helix ha one strand of a DNA double helix has the sequence AGTACTG, what will be the sequence of the other strand? A) GACGTCA B) AGTACTGC) GTCATGAD) TCATGAC 49) A DNA molecule has the sequence AGTTCAACT. The equivalent RNA molecule would have the sequence AGTTCAACT B) AGUUCAACU C) UGTTCUUCT D) UGUUCUUCU 50) what group(s) in amino acid carboxyl and Amino D) Hydroxyl and Carboxyl B) carbonyl and Amino C) Hydroxyl and Carbonyl
Transcription is the process of rewriting DNA. DNA molecules are made from 4 different nucleotides which...
Transcription is the process of rewriting DNA. DNA molecules are made from 4 different nucleotides which acts as building blocks. These building blocks are adenine, thymine, guanine and cytosine. When put together in different chemical combinations they become directions for the functions of the cell molecules which are primarily proteins. When a certain protein is needed, the RNA polymerase enzyme will find the gene for that particular protein and makes an RNA copy of it. DNA and RNA have similar...
If the sequence of the 5'-3'DNA strand is AATGCTAC, then the complementary DNA sequence has the...
If the sequence of the 5'-3'DNA strand is AATGCTAC, then the complementary DNA sequence has the following sequence o 3-AATGCTAC-5' 3'-CATCGTAA-5 3-GTAGCATT-5 3-TTACGATG-5 Question 2 20 pts Which of the following does the enzyme primase make? phosphodiester linkages (bonds) Okazaki fragments RNA primer DNA primer In which direction does DNA replication take place? 3'to 5 5'to 5 5'to 3 3'to 3 Question 4 20 pts Which enzyme unwinds the double-stranded helical DNA starting at the origin of replication? ligase primase...
3. The partial gene (DNA) sequence below contains multiple PAM sequences. Highlight six PAM sequences in...
3. The partial gene (DNA) sequence below contains multiple PAM sequences. Highlight six PAM sequences in the top (5' to 3) strand. 5'-GCACGGCGGAGCGGTTCTTGGCAGCGGCCGCACGATCTCGTTGCCGCCGG- 3' 3-CGTGCCGCCTCGCCAAGAACCGTCGCCGGCGTGCTAGAGCAACGGCGGCC- 5' Once Cas9 binds to a PAM sequence, it unwinds the DNA. If the guide RNA matches the DNA sequence next to the PAM, the guide RNA will bind to the complementary DNA strand. If not, the DNA will zip back together and Cas9 will keep binding to other PAM sequences until it finds the...
please help me with 6 and 7
sorry 7 and 8
Pyruvate. Km = 400 micromolar HCO3 km = 1000 micromolar ATP. Km = 60 micromolar 7 Which protein sequence is mostly likely to undergo phosphorylation in a cell? 0 (2 points) GRTGTK GALNO GWPDE GCRKK 8 If you see a DNA sequence such as ATGGC, which base makes the 3' end? Which base/bases has a free hydroxyl group? If this was RNA (AUGGC), which base/bases have a free hydroxyl...
Review. Consider how one might label the 5'ends of DNA for a footprinting experiment where you visualize where on the DNA a transacting protein binds to the cis acting promoter sequence The 5 phosphate(s) at the S'ends a DNA can be removed using a phosphatase, like calf intestinal phosphatase (CIP), in vitro, leaving a 5-hydroxyl group at the end of DNA CIP has no impact on the phosphates along the backbone of the DNA, just the 5' end phosphate and...
Unwinds DNA strand to make replication fork. Adds free nucleotides to the growing daughter DNA strands Adds short pieces of RNA to help DNA polymerase start Removes RNA and replaces with DNA Fuses or "glues" fragments of DNA together Proofreads or edits the DNA, checking for mistakes Given the following, DNA Sequence, what is the new daughter strand? (Did you label the 5' and 3' ends?) What is the name of the "fragments" of DNA on the lagging strand after...
the several other 10.4 to show t 3. The base uracil substitutes for the base thymine in RNA. Complete Table ways RNA differs from DNA Table 10.4 DNA Structure Compared with RNA Structure RNA Sugar Bases Strands Helix DNA Deoxyribose Adenine, guanine, thymine, cytosine Double stranded with base pairing Yes Complementary Base Pairing Complementary base pairing occurs between DNA and RNA. The RNA base uracil pairs with the DNA base adenine; the other bases pair as shown previously. Complete Table...
48) If one strand of a DNA double helix ha one strand of a DNA double helix has the sequence AGTACTG, what will be the sequence of the other strand? A) GACGTCA B) AGTACTGC) GTCATGAD) TCATGAC 49) A DNA molecule has the sequence AGTTCAACT. The equivalent RNA molecule would have the sequence AGTTCAACT B) AGUUCAACU C) UGTTCUUCT D) UGUUCUUCU 50) what group(s) in amino acid carboxyl and Amino D) Hydroxyl and Carboxyl B) carbonyl and Amino C) Hydroxyl and Carbonyl
If the sequence of the 5'-3'DNA strand is AATGCTAC, then the complementary DNA sequence has the following sequence o 3-AATGCTAC-5' 3'-CATCGTAA-5 3-GTAGCATT-5 3-TTACGATG-5 Question 2 20 pts Which of the following does the enzyme primase make? phosphodiester linkages (bonds) Okazaki fragments RNA primer DNA primer In which direction does DNA replication take place? 3'to 5 5'to 5 5'to 3 3'to 3 Question 4 20 pts Which enzyme unwinds the double-stranded helical DNA starting at the origin of replication? ligase primase...
3. The partial gene (DNA) sequence below contains multiple PAM sequences. Highlight six PAM sequences in the top (5' to 3) strand. 5'-GCACGGCGGAGCGGTTCTTGGCAGCGGCCGCACGATCTCGTTGCCGCCGG- 3' 3-CGTGCCGCCTCGCCAAGAACCGTCGCCGGCGTGCTAGAGCAACGGCGGCC- 5' Once Cas9 binds to a PAM sequence, it unwinds the DNA. If the guide RNA matches the DNA sequence next to the PAM, the guide RNA will bind to the complementary DNA strand. If not, the DNA will zip back together and Cas9 will keep binding to other PAM sequences until it finds the...
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