What is the purpose in a PCR reaction for each of the following reagents? Taq Taq...
- What is the purpose in a PCR reaction for each of the
following reagents?
- Taq
- Taq buffer
- dNTPs
- Forward primer
- Reverse primer
- Genomic DNA
- What do you think would happen if you forgot to add your Reverse primer when you did a PCR?
Homework Answers
A- taq - the polymerase enzyme help in adding the nucleotide to the primer and forming chain
taq buffer maintain the ph of the reaction
dntp are added as substrate for dna molecule
forward primer is utlilized in leading strand and revrse primer is utilized in lagging strand . Genomic dna is used as template for replication
the absence of reverse primer lead to no replication on lagging strand.
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What is the purpose in a PCR reaction for each of the
following reagents?
Taq
Taq...
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2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
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Order the steps in RT-PCR: 1. Add one primer complementary to the 3' end of the...
Order the steps in RT-PCR: 1. Add one primer complementary to the 3' end of the RNA of interest. Also add the RT enzyme (reverse transcriptase), dNTPs, and a buffer with Mg2+. Incubate the reaction appropriately. 2. Degrade the RNA strand with base, leaving just the cDNA. 3. cDNA is produced, which is single-stranded and complementary to the RNA of interest. 4. Double-stranded DNA for the region of interest is produced. 5. Add two primers (one forward and one reverse)...
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what do you think she should change in her protocol to avoid
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2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
2. Design a PCR experiment to amplify a sequence of interest. Use the following reagent concentrations for calculating your cocktail. Fill in the chart below. A. Fill in the chart below with the volumes that would be added to each tube. Note the final volume of the reaction. Show your calculations for full credit. REAGENT Final Concentration (50 ul Reaction) 1x Test Reaction (+) Control Reaction 10x Reaction Buffer ML dNTPs (15 mm) 200 UM 0.2 UM ul 0.2 um...
biochemistry question
Nucleic Acids 33 boslari, no NO E Let's Make a PCR Reaction Reaction Mixture: You have stock DNA of 100 ng/uL that you wish to amplify. You have stocks of 10X PCR Buffer, 10 uM of the forward and reverse primers, 25 mM MgCl2, 50 mM dNTPs , and Tag that is 10 Units/UL. Create the Recipe for a 20 PL total PCR that uses 50 ng of DNA, 2.5 mM primers, 5 mM MgCl2, 5 mM dNTPs,...
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Problem.3: A student was working on PCR and she performs the additives shown in (A) and get the result in (B): B. Component Volume per reaction (ul) 10X PCR buffer 2.5 MgSO4 dNTPs (10mM) Enzyme Forward Primer (10 pmale) BCH361 (Practicall Biochemistry department Reverse Primer (10 pmele) RNase/DNase-free water 14.8 DNA 4 ul Total volume 25
Which of the following molecules is not required for a PCR reaction? View Available Hint(s) Ligase O Primer O DNA O DNTPs Submit Previous Answers X Incorrect; Try Again; One attempt remaining dNTPs are required because they are the building blocks of DNA molecules. - Part B The thermostability of Taq polymerase is required during the annealing phase of PCR. View Available Hint(s) O True O False Submit
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