3. Amino acid analysis of a peptide seven residues long gave: Asp, Leu, Lys,...
3. Amino acid analysis of a peptide seven residues long gave:
Asp, Leu, Lys, Met, Phe, Tyr
The following facts were observed:
a. Trypsin treatment had no apparent effect
b. Reaction with phenylisothiocyanate reagent yielded PTH-Phe
c. Treatment with chymotrypsin yielded several products, including a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lys and Met.
d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and free lysine.
What is the amino acid sequence of this heptapeptide?
Homework Answers
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3. Amino acid analysis
of a peptide seven residues long gave:
Asp, Leu, Lys,...
Styles A decapeptide has the following amina acid composition: Arg. Asp, Gly, Leu, Lys, Met, Phe,...
Styles A decapeptide has the following amina acid composition: Arg. Asp, Gly, Leu, Lys, Met, Phe, Ser. Trp, and Val Reacting the native peptide with FDNB and then hydrolyzing released 2.4- dinitrophenylvaline. Brief incubation of the native peptide with carboxypeptidase yielded free Leu. Incubation with cyanogen bromide yielded two fragments: a tetrapeptide with composition Met, Phe, Ser, and Val, and a hexapeptide. The hexapeptide yielded 2.4- dinitrophenylglycine. Proteolytic cleavage by trypsin of the native peptide gave free Leu, a tripeptide,...
1. Partial hydrolysis of lysozyme yielded an octapeptide that was later identified as the N-terminal segment of...
1. Partial hydrolysis of lysozyme yielded an octapeptide that was later identified as the N-terminal segment of the protein. From the following information, determine the sequence of this octapeptide. a. The following amino acids were identified after complete hydrolysis of the octapeptide: Arginine (Arg) Glycine (Gly) Phenylalanine (Phe) Cysteine (Cys) Leucine (Leu) Valine (Val) Glutamic Acid (Glu) Lysine (Lys) b. Treatment of the octapeptide with dinitroflurobenzene (Sanger reagent) followed by complete hydrolysis gave lysine (Lys) labeled with two dinitrophenyl (DNP) groups. c. Treatment of the octapeptide with trypsin gave lysine...
anyone can help me with it? A protein biochemist attempted to determine the amino acid sequence...
anyone can help me with it?
A protein biochemist attempted to determine the amino acid sequence of a decapeptide. Use the results from the trypsin, chymotrypsin, and cyanogen bromide treatments to suggest the amino acid sequence of this decapeptide. Trypsin digestion gave two fragments with multiple residues (not in order): • T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr • T2: Lys, Met, Val Chymotrypsin digestion gave four fragments with multiple residues (not in order): • CT1: Ala, Phe •...
biochemistry please show your work 2. A mutant form of polypeptide hormone angiotensin II has the...
biochemistry please show your work
2. A mutant form of polypeptide hormone angiotensin II has the amino acid composition: Ile, Arg, Met, Leu, Phe, Asp, Tyr, and Val. The following observations are made: 1) Treatment with trypsin yields a dipeptide containing Asp and Arg, and a hexapeptide with all the rest. 2) Cyanogen bromide yields a dipeptide containing Phe and Leu and a hexapeptide containing all the rest. 3) Chymotrypsin cleaves the hormone into two tetrapeptides of composition: Val, Tyr,...
A heptapeptide was found to hawe an anino acid composition of asp leu, lys, met.phe and...
A heptapeptide was found to hawe an anino acid composition of asp leu, lys, met.phe and tyr Trypsuin has no efect on the heptapeptide One cycle of Edman degradation randers the product whose structurs is shown below Chymatrypain treatment yields a dpapede and a tetrapaplide, as well as a bee amne acid The tetrapeptide is known to contain asp, leu lys, and met. Cyanagen bromde treatment generates a dpeptide that is determined to ariginate frone the N-taminus af the heptapetide,...
please help!!! 19. A peptide is composed of the following amino acids: Ala, Arg. Glu, Gly,...
please help!!!
19. A peptide is composed of the following amino acids: Ala, Arg. Glu, Gly, Lys, Met, Phe, Ser, Tyn Given the following data deduce the sequence of the peptide. Trypsin digestion yielded two fragments with the following composition: Fragment 1: Ala, Arg, Glu, Met, Phe, Ser Fragment 2: Gly, Lys, Tyr Chymotrypsin digestion yielded three fragments with the listed compositions: Fragment 1: Ala, Gly, Lys, Phe Fragment 3: Arg, Glu, Met, Ser Cyanogen Bromide cleavage yielded two fragments...
How to do this problem? 1.A 14 AA peptide contains: Ala, Arg,, Gly, His, Leu, Lys, Phez, Serz, Trp, Tyr, Val This pe...
How
to do this problem?
1.A 14 AA peptide contains: Ala, Arg,, Gly, His, Leu, Lys, Phez, Serz, Trp, Tyr, Val This peptide yields the following results when broken down into smaller fragments Please note the AA are given in alphabetical order). Show your work, explain your reasoning, give the answer in three letter abbreviation and one letter abbreviation Fragment Content His Treatment Edman Chymotrypsin Gly. His, Phe Ser, Tyr Arg2, Leu, Lys, Ser.Ala, Phe Trp, Val Gly, His, Lys,...
Question 13 of 19 > Suppose Kara has isolated a peptide and must determine the amino...
Question 13 of 19 > Suppose Kara has isolated a peptide and must determine the amino acid sequence. After one cycle of Edman degradation on a sample of the peptide, she produces a phenylthiohydantoin (PTH) derivative, OH Treatment with cyanogen bromide on another sample of her peptide gives her the fragments • Lys-Pro-Ala-Met, • Pro-Asp. • Leu-Ile-Cys-Trp-Met, and • Thr-Arg.Met. She also digested a sample of her peptide with the enzyme chymotrpsin. One of the fragments produced after treatment with...
5. Consider the following peptide: His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gin- Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr a. What are the fragments, if it is cleaved...
5. Consider the following peptide: His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gin- Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr a. What are the fragments, if it is cleaved by trypsin? b. What are the fragments, if it is cleaved by chymotrypsin? c. What are the fragments, if it is cleaved by pepsin?
How to do this problem? 1.A 14 AA peptide contains: Ala, Arg,, Gly, His, Leu, Lys,...
How
to do this problem?
1.A 14 AA peptide contains: Ala, Arg,, Gly, His, Leu, Lys, Phez, Serz, Trp, Tyr, Val This peptide yields the following results when broken down into smaller fragments Please note the AA are given in alphabetical order). Show your work, explain your reasoning, give the answer in three letter abbreviation and one letter abbreviation Fragment Content His Treatment Edman Chymotrypsin Gly. His, Phe Ser, Tyr Arg2, Leu, Lys, Ser.Ala, Phe Trp, Val Gly, His, Lys,...
Styles A decapeptide has the following amina acid composition: Arg. Asp, Gly, Leu, Lys, Met, Phe, Ser. Trp, and Val Reacting the native peptide with FDNB and then hydrolyzing released 2.4- dinitrophenylvaline. Brief incubation of the native peptide with carboxypeptidase yielded free Leu. Incubation with cyanogen bromide yielded two fragments: a tetrapeptide with composition Met, Phe, Ser, and Val, and a hexapeptide. The hexapeptide yielded 2.4- dinitrophenylglycine. Proteolytic cleavage by trypsin of the native peptide gave free Leu, a tripeptide,...
anyone can help me with it?
A protein biochemist attempted to determine the amino acid sequence of a decapeptide. Use the results from the trypsin, chymotrypsin, and cyanogen bromide treatments to suggest the amino acid sequence of this decapeptide. Trypsin digestion gave two fragments with multiple residues (not in order): • T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr • T2: Lys, Met, Val Chymotrypsin digestion gave four fragments with multiple residues (not in order): • CT1: Ala, Phe •...
biochemistry please show your work
2. A mutant form of polypeptide hormone angiotensin II has the amino acid composition: Ile, Arg, Met, Leu, Phe, Asp, Tyr, and Val. The following observations are made: 1) Treatment with trypsin yields a dipeptide containing Asp and Arg, and a hexapeptide with all the rest. 2) Cyanogen bromide yields a dipeptide containing Phe and Leu and a hexapeptide containing all the rest. 3) Chymotrypsin cleaves the hormone into two tetrapeptides of composition: Val, Tyr,...
A heptapeptide was found to hawe an anino acid composition of asp leu, lys, met.phe and tyr Trypsuin has no efect on the heptapeptide One cycle of Edman degradation randers the product whose structurs is shown below Chymatrypain treatment yields a dpapede and a tetrapaplide, as well as a bee amne acid The tetrapeptide is known to contain asp, leu lys, and met. Cyanagen bromde treatment generates a dpeptide that is determined to ariginate frone the N-taminus af the heptapetide,...
please help!!!
19. A peptide is composed of the following amino acids: Ala, Arg. Glu, Gly, Lys, Met, Phe, Ser, Tyn Given the following data deduce the sequence of the peptide. Trypsin digestion yielded two fragments with the following composition: Fragment 1: Ala, Arg, Glu, Met, Phe, Ser Fragment 2: Gly, Lys, Tyr Chymotrypsin digestion yielded three fragments with the listed compositions: Fragment 1: Ala, Gly, Lys, Phe Fragment 3: Arg, Glu, Met, Ser Cyanogen Bromide cleavage yielded two fragments...
How
to do this problem?
1.A 14 AA peptide contains: Ala, Arg,, Gly, His, Leu, Lys, Phez, Serz, Trp, Tyr, Val This peptide yields the following results when broken down into smaller fragments Please note the AA are given in alphabetical order). Show your work, explain your reasoning, give the answer in three letter abbreviation and one letter abbreviation Fragment Content His Treatment Edman Chymotrypsin Gly. His, Phe Ser, Tyr Arg2, Leu, Lys, Ser.Ala, Phe Trp, Val Gly, His, Lys,...
Question 13 of 19 > Suppose Kara has isolated a peptide and must determine the amino acid sequence. After one cycle of Edman degradation on a sample of the peptide, she produces a phenylthiohydantoin (PTH) derivative, OH Treatment with cyanogen bromide on another sample of her peptide gives her the fragments • Lys-Pro-Ala-Met, • Pro-Asp. • Leu-Ile-Cys-Trp-Met, and • Thr-Arg.Met. She also digested a sample of her peptide with the enzyme chymotrpsin. One of the fragments produced after treatment with...
5. Consider the following peptide: His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gin- Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr a. What are the fragments, if it is cleaved by trypsin? b. What are the fragments, if it is cleaved by chymotrypsin? c. What are the fragments, if it is cleaved by pepsin?
How
to do this problem?
1.A 14 AA peptide contains: Ala, Arg,, Gly, His, Leu, Lys, Phez, Serz, Trp, Tyr, Val This peptide yields the following results when broken down into smaller fragments Please note the AA are given in alphabetical order). Show your work, explain your reasoning, give the answer in three letter abbreviation and one letter abbreviation Fragment Content His Treatment Edman Chymotrypsin Gly. His, Phe Ser, Tyr Arg2, Leu, Lys, Ser.Ala, Phe Trp, Val Gly, His, Lys,...
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