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Question 13 of 19 > Suppose Kara has isolated a peptide and must determine the amino acid sequence. After one cycle of Edman
Treatment with cyanogen bromide on another sample of her peptide gives her the fragments • Lys-Pro-Ala-Met. • Pro-Asp. • Leu-
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Answer #1

Cyanogen bromide cleaves at C- terminal of methionine residues. Thus the second peptide must be in the last of the sequence because it does not have any methionine residue. Chymotrypsin cleaves C- terminal of aromatic amino acid that are W, Y, and F. So the correct order of amino acids/ peptides in this protein will be-

TRM-KPAM-LICTM-PE

Where all single letter codes are taken for the peptide here. Chymotrypsin will cleave at carboxy-terminus of tryptophan and a fragment of MPE leaves behind.

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