Is it possible to sequence rRNA directly, that is, using the ribosome rather than the DNA...
Is it possible to sequence rRNA directly, that is, using the ribosome rather than the DNA from the nucleus? For example, this paper, Complete nucleotide sequence of a 16s rRNA gene from E. coli, suggests that it uses DNA to find the sequence. Would it be possible to isolate the rRNA and derive the sequence from that?
Homework Answers
As far as I know it'spossible to reverse transcribe a rRNA gene, you may not get the best yields though. The secondary / tertiary structures will be a problem, however, there are reverse transcriptase's commercially available that can handle secondary structure: Thermo-X
It is not possible to sequence rRNA directly from ribosomes. For such to done, primarily ribosomes have to be separated from the remaining cell organelles. Ribosomes are very complex structures with a mixture of proteins and rRNA in it. All the rRNAs present in it are linked with some proteins. Therefore, it is highly difficult to extract purely rRNA from that mixture. In contrast, extraction of DNA from the nucleus is quite easier and rRNA can be sequenced using it.
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Is it possible to sequence rRNA directly, that is, using the
ribosome rather than the DNA...
Once a bacterial 16s rRNA gene has been amplified, it is possible to have the nucleotide...
Once a bacterial 16s rRNA gene has been amplified, it is possible to have the nucleotide sequence of the DNA determined. There are many different techniques for this. In lab, you isolated and amplified DNA from a known organism (Escherichia coli), but what if you had to identify an unknown organism? You would send the sample away for sequencing and then once you got the results you would “BLAST” the sequence. Using the Standard Nucleotide BLAST on NCBI and the...
1) Using the bacterial DNA sequence that the instructor gave you:
1) Using the bacterial DNA sequence that the instructor gave you:a. Identify and underline the promoter region and the start codon.b. Identify the coding and template strandc. Transcribe the coding sequenced. Translate the mRNA sequence8) Which of the following mutational changes would you predict to be the most deleterious to gene function? Explain your answers.a. Insertion of a single nucleotide near the end of the coding sequence.b. Removal of a single nucleotide near the beginning of the coding sequence.c. Deletion...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence,...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence, the tRNA anticodons, and the amino acid sequences (use the three letter code) that would result from it. (3 points) DNA +1 15'GICIA I G C A A CICATI I AA GG 3' 3" CA GATA C GTIGA GIA A A IICC 5 mRNA tRNA anticodons amino acids 7. You are interested in a gene that codes for a 20 amino acid-long protein. (1.5...
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A....
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A. Only ribonucleoside triphosphates contain the sugar ribose. B. Ribonucleoside triphosphates have low potential energy, making the polymerization reaction endergonic. C. Ribonucleoside triphosphates have high potential energy, making the polymerization reaction exergonic. D. Ribonucleoside monophosphates cannot form complementary base pairs with the DNA template. E. Ribonucleoside triphosphates are not used, rather all use deoxyriboside triphosphates. 14. How is a mutation in a bacterial cell that...
TASK II DNA Sequence DNA was also collected (Table 1). You are going to use this...
TASK II DNA Sequence DNA was also collected (Table 1). You are going to use this DNA sequence to complete the assignment Sacher, tochemelebiT.CO. DA A CARTIE CURIE CRET GARAGE MATIC I GREW E G CARETTING GUITAR HET GRATUIT TE GOOIT GENIET CARTIE CUCINE ACRET GARAGE GOTE GOOIT GRONIE I The DNA examined is 100 nucleotides of mitochondrial sequence, specifically the cytochrome Coxidase subunit I (MT-001) gene. This gene is often used as a DNA barcode to identity/differentiate animals at...
Shown below is the anti-sense DNA sequence from a region of a gene that produces a...
Shown below is the anti-sense DNA sequence from a region of a gene that produces a specific protein. Mutations in this region of the gene cause a disease CTT TTA TAG TAG ATA CCA CAA AGG a. What is the mRNA strand that is transcribed from the DNA shown above? b. What is the amino acid sequence that would be translated from the mRNA strand you determined in part 1? c. If an individual has a G at position 15...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
The individual subunits making up each strand of DNA are called1 Inherited traits are determined by...
The individual subunits making up each strand of DNA are called1 Inherited traits are determined by elements of heredity called__2 . 3 __ in the base sequence to a DNA Transcription is a production of an RNA strand that is_ strand. In eukaryotes RNA_ 4 synthesizes mRNA, located in the The main concept in the 6 of molecular biology is that DNA does not code for protein directly but rather acts through an intermediary molecule of ribonucleic acid (RNA). An...
5. Genetics. Please provide complete, correct and verified solution done on computer or by hand. This...
5.
Genetics. Please provide complete, correct and verified solution
done on computer or by hand. This is all the information provided.
Please only provide answers. Thank you very much.
positions would you expecr 16. The figure below shows a replication fork in E. coli.At which of the indicated positions would you expect to see a 3'OH? A. B. D. E. A and B 17. Select the TRUE statement. E. coli mRNA A. is produced from both DNA template strands at...
2. When transcribing an mRNA strand, RNA polymerase uses the strand of DNA to match complementary...
2. When transcribing an mRNA strand, RNA polymerase uses the strand of DNA to match complementary bases with. RNA polymerase always reads this strand in the direction and always builds mRNA in the direction. (1.5 pts) 3. (0.5 pt) What is the significance of the +1 site in regards to transcription of mRNA? t) When translating an mRNA sequence, where does the ribosome always begin? 5. (0.5 pt) When translating an mRNA sequence, what signals the ribosome to end translation?...
1) Using the bacterial DNA sequence that the instructor gave you:a. Identify and underline the promoter region and the start codon.b. Identify the coding and template strandc. Transcribe the coding sequenced. Translate the mRNA sequence8) Which of the following mutational changes would you predict to be the most deleterious to gene function? Explain your answers.a. Insertion of a single nucleotide near the end of the coding sequence.b. Removal of a single nucleotide near the beginning of the coding sequence.c. Deletion...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence, the tRNA anticodons, and the amino acid sequences (use the three letter code) that would result from it. (3 points) DNA +1 15'GICIA I G C A A CICATI I AA GG 3' 3" CA GATA C GTIGA GIA A A IICC 5 mRNA tRNA anticodons amino acids 7. You are interested in a gene that codes for a 20 amino acid-long protein. (1.5...
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A. Only ribonucleoside triphosphates contain the sugar ribose. B. Ribonucleoside triphosphates have low potential energy, making the polymerization reaction endergonic. C. Ribonucleoside triphosphates have high potential energy, making the polymerization reaction exergonic. D. Ribonucleoside monophosphates cannot form complementary base pairs with the DNA template. E. Ribonucleoside triphosphates are not used, rather all use deoxyriboside triphosphates. 14. How is a mutation in a bacterial cell that...
TASK II DNA Sequence DNA was also collected (Table 1). You are going to use this DNA sequence to complete the assignment Sacher, tochemelebiT.CO. DA A CARTIE CURIE CRET GARAGE MATIC I GREW E G CARETTING GUITAR HET GRATUIT TE GOOIT GENIET CARTIE CUCINE ACRET GARAGE GOTE GOOIT GRONIE I The DNA examined is 100 nucleotides of mitochondrial sequence, specifically the cytochrome Coxidase subunit I (MT-001) gene. This gene is often used as a DNA barcode to identity/differentiate animals at...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
The individual subunits making up each strand of DNA are called1 Inherited traits are determined by elements of heredity called__2 . 3 __ in the base sequence to a DNA Transcription is a production of an RNA strand that is_ strand. In eukaryotes RNA_ 4 synthesizes mRNA, located in the The main concept in the 6 of molecular biology is that DNA does not code for protein directly but rather acts through an intermediary molecule of ribonucleic acid (RNA). An...
5.
Genetics. Please provide complete, correct and verified solution
done on computer or by hand. This is all the information provided.
Please only provide answers. Thank you very much.
positions would you expecr 16. The figure below shows a replication fork in E. coli.At which of the indicated positions would you expect to see a 3'OH? A. B. D. E. A and B 17. Select the TRUE statement. E. coli mRNA A. is produced from both DNA template strands at...
2. When transcribing an mRNA strand, RNA polymerase uses the strand of DNA to match complementary bases with. RNA polymerase always reads this strand in the direction and always builds mRNA in the direction. (1.5 pts) 3. (0.5 pt) What is the significance of the +1 site in regards to transcription of mRNA? t) When translating an mRNA sequence, where does the ribosome always begin? 5. (0.5 pt) When translating an mRNA sequence, what signals the ribosome to end translation?...
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