Question

Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the proper proteins to transcribe the eukaryotic gene. The gene will induce attenuation in the bacterial cell, which will cause a delay in the transcription and translation of the hGH gene. The bacterial RNA polymerase will not bind to the eukaryotic promoter sequence.

Answer 1

The eukaryotes gene contains introns in the gene they are not able to process inside the bacteria. Two genes are separated by introns in the eukaryotes and both genes has to express to make a final functional protein. But in vector we only place one promoter and place our gene of interest and it express only one gene and stop at intron region.Also bacteria RNA polymerase not binds to eukaryotic promoter.

Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the proper proteins to transcribe the eukaryotic gene. The gene will induce attenuation in the bacterial cell, which will cause a delay in the transcription and translation of the hGH gene. The bacterial RNA polymerase will not bind to the eukaryotic promoter sequence.

Order the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for hGH. Not all steps will be placed. Continued below... Isolate the human gene that produces hGH Isolate the mRNA of the hGH gene. Generate cDNA of the hGH gene The order is correct using reverse transcriptase. Insert the hGH cDNA into a bacterial vector near a promoter site. Transform the vector into an E. coli bacterium E. coli expresses hGH. Correct Human DNA is introduced into the bacterial cell using direct injection by a small needle The hGH protein is isolated from human tissues.