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Explain how you might engineer E. coli to produce human growth hormone (HGH)...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
Below is a set of experiments for cloning the human growth hormone (HGH) gene and then...
Below is a set of experiments for cloning the human growth hormone (HGH) gene and then expressing the HGH protein in E. coli starting from human pituitary glands and a tube of plasmid vector DNA. The plasmid expression vector contains the arabinose inducible expression system. Specify the correct order for carrying out these experiments, using the letter codes in front of each procedure. Use all of the steps in your answer, except for one step that should NOT be included....
the genetic blueprint of an organism is referred to as i A scientist wishes to genetically...
the genetic blueprint of an organism is referred to as i
A scientist wishes to genetically engineer E. coli bacteria so that it will contain the green fluorescent protein (GFP) gene. Which of the following protocol outlines would the scientist use to accomplish this task? ОА Insert GFP gene into corn expression cassette; insert expression cassette into plasmid vector; insert plasmid vector into Agrobacterium tumefaciens bacteria; allow bacteria to infect corn plant; GFP gene inserted into com cell genome. ....
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
In a hypothetical scenario. You wake up one morning to find your hah has suddenly grown....
In a hypothetical scenario. You wake up one morning to find your hah has suddenly grown. YOU have been supplementing your diet with a strange now fungus purchased at the local farmer's market. You take samples of the fungus to your lab and you find that this fungus does indeed make a protein (the her E protein) that stimulates hair growth. Yon con struct a fungal genomic DNA library in E. Coli with the hope of cloning the hart gene....
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into a tool for biotechnology? a. It could possibly be turned into a viral vector against lung cancers b. Its promoters might be used to express genes in lung cells c. Its surface proteins could be used for new epitope tags d. All of the above QUESTION 2 Which of the following are applications of molecular assembly described in this course? a. It can be...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
the genetic blueprint of an organism is referred to as i
A scientist wishes to genetically engineer E. coli bacteria so that it will contain the green fluorescent protein (GFP) gene. Which of the following protocol outlines would the scientist use to accomplish this task? ОА Insert GFP gene into corn expression cassette; insert expression cassette into plasmid vector; insert plasmid vector into Agrobacterium tumefaciens bacteria; allow bacteria to infect corn plant; GFP gene inserted into com cell genome. ....
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
In a hypothetical scenario. You wake up one morning to find your hah has suddenly grown. YOU have been supplementing your diet with a strange now fungus purchased at the local farmer's market. You take samples of the fungus to your lab and you find that this fungus does indeed make a protein (the her E protein) that stimulates hair growth. Yon con struct a fungal genomic DNA library in E. Coli with the hope of cloning the hart gene....
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