Question

Protein Name is : Concanavalin – A Uniprot entry number is : P02866

• Search the literature to source the original discovery route for this protein.

external website :  http://www.uniprot.org/uniprot/P02866/publications

• Present a summary for the first purification carried out on this protein as a simplified flow chart. It must present a summary overview of the chemical partitioning events, not detailed methods & reagents. Clearly label discarded and retained fractions at each partition.

•Give a detailed description of the molecular events and chemistry of each separation step in the specific procedure

. • Where possible, include and discuss column traces or stained gel images that indicate the purification achieved at specific steps.

• consistent bibliography style and use of references is required .

Answer 1

The protein corresponding to the UniProt ID: P02866 is Concanavalin-A

produced by a plant species Canavalia ensiformia (Jack beans)

It is a carbohydrate binding protein (Lectin) and specifically binds to D-mannose.

Moreover, it binds to the transitional metal ions like manganese (Mn) and also calcium (Ca)

the summary of its first purification is as follows Edelman GM, Cunningham BA, Reeke GN, Becker JW, Waxdal MJ, Wang JL. The Covalent and Three-Dimensional Structure of Concanavalin A. Proceedings of the National Academy of Sciences of the United States of America. 1972;69(9):2580-2584.:

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was con duched on Bio-Rad Aa50Ax chvom a (30-35)muintaine d a o gx 15cm) pHろーリ ynidina cidjuskd to ph s-S Linear crya dent was ure d with ace hcaud to 2 0M by adding acahc aud Smull co lumn 1 SO 200 ml bu Larye る50 ml -low rat 30 o mr was mm aint ainad by mini Pumb ere Se bha he colteted