Answer:
The lac repressor is a protein that inhibits the transcription of the lac operon. It does this by binding to the operator, which partially overlaps with the promoter. When bound, the lac repressor gets in RNA polymerase's way and keeps it from transcribing the operon. whareas, un avaibility of lactose in the cell, the lac repressor binds tightly to the operator, preventing transcription by RNA polymerase. Therefore, when lactose is present, the lac repressor loses its ability to bind DNA. It floats off the operator, clearing the way for RNA polymerase to transcribe the operon. So, from the above figure clear that the binding behaviour of Polymerase are simliar in fasion as Lac repressor in presence of lactose.
How is the DNA-binding behavior of polymerase similar to that of the lac repressor?
How does the Trp repressor prevents RNA polymerase from initiating transcription? by blocking the ribosome binding site by forming a loop in the operator that restricts the passage of the polymerase by binding to the polymerase to inhibit its catalytic activity by physically blocking the DNA binding site of RNA polymerase
Bacteria are logic machines Input Output DNA-Binding Lacl CRP Lac operone |(repressor)| (activator) | Glucose + + Lactose + I
The lac repressor active site The active site of the lac repressor protein is composed of OK Multiple Choice Inces O the protein domain that binds to allolactose. the protein domain that binds to DNA the protein domain that binds to other lac repressors O a glucose binding domain < Prev
How is it possible for the repressor to enhance binding of RNA polymerase to the promoter while at the same time repressing transcription?
a) For the lac operon, will the repressor or RNA polymerase be bound to the operon in this situation? Draw what will be happening on the operon below. PROMOTER OPERATOR Lactose Enzyme 1 Lsctose Enzyme2 Lactose Enzyme 3 NO b) Will transcription occur? c) Describe what is happening (with vocabulary words). YES 2. Bobby Joe is fasting today, how will the E. coli in her stomach respond to the lack of Tryptophan? a) For the trp operon, will the repressor...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
1. How do prokaryotes conserve energy? 2. How do DNA-binding proteins in prokaryotes regulate genes? 3. What is an operon? 4. What is in the lac operon in E. coli? 5. What is the function of the genes in the lac operon of E. coli? 6. What turns the lac operon off? 7. How does a repressor protein turn off the lac operon? 8. How does lactose turn on the lac operon?
In the lac operon, 1 codes for the repressor protein, P is the promoter which is necessary for binding RNA polymerase, O is the operator, and Z codes for the B-galactosidase. Assuming (+) denotes the wild type for a particular locus, (-) denotes a lack of a particular gene product or function, O^c is a constitutive operator, and I^s is a super repressor. Use a "+++" sign to indicate that significant quantities of functional B-galactosidase are present, a '+' sign...
The repressor protein for the Gal1 locus (does not / does) contain a DNA binding domain.
21. Double helix22. Repressor protein23. Adenine24. Ribosome.25. Promoter26. Replication27. RNA Polymerase.28. CodonA. Enzyme that synthesizes RNAB. Organelle where proteins are assembledC. Complementary to either Thymine or UracilD. mRNA sequence that codes for one amino acidE. Shape of double stranded DNAF. Sequence of DNA that controls gene expressionG. binds an operator and stops gene expression in LAC operon by preventing RNA polymerase from binding gene and transcribing. H. Duplication of DNA in 5 phase of Interphase