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Bacteria are logic machines Input Output DNA-Binding Lacl CRP Lac operone |(repressor)| (activator) | Glucose +...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
Consider what you know about the lac operon, and predict whether the repressor and the activator...
Consider what you know about the lac operon, and predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels of transcription. and the activator is b. When lactose and glucose are...
Consider an E. coli strain carrying a mutation in crp (encoding the catabolite activator protein) that...
Consider an E. coli strain carrying a mutation in crp (encoding the catabolite activator protein) that prevents CAP from binding CAMP. Predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels...
for 1-5 define those The Lac operon is an inducible set of genes found in bacteria...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
Mention the components of bacterial transcriptional switches. The Lac operon is regulated by both activator CAP...
Mention the components of bacterial transcriptional switches. The Lac operon is regulated by both activator CAP and Lac repressor. A scientist introduces a mutation in the gene encoding the activator CAP, making this allosteric protein insensitive to glucose levels. What outcomes do you expect if you culture the mutated bacteria in a culture medium with glucose and lactose. Give your explanation based on the mechanism of the Lac operon.
How is the DNA-binding behavior of polymerase similar to that of the lac repressor?
How is the DNA-binding behavior of polymerase similar to that of the lac repressor?
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation...
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation test. The first F' plasmid contains the entire lac operon from a strain that is lacz, while the second F' plasmid contains the entire lac operon from a strain that is lacY. You test the ability of the transformed bacteria to grow on media where the sole carbon source is either glucose or lactose, and obtain the following results: Lac t mutant compl lacZ...
alllll them please all To MOST readily demonstrate transformation of bacteria in the laboratory one could...
alllll them please all
To MOST readily demonstrate transformation of bacteria in the laboratory one could extract DNA from: A) his cells and add the DNA to his cells, then grow the cells on plates with histidine B) hist cells and add the DNA to his cells, then grow the cells on plates without histidine C) lac" cells and add the DNA to lact cells, then grow the cells on plates without histidine D) amp cells and add the DNA...
all them please Question 23 (1 point) The A and B alleles in ABO blood types...
all them please
Question 23 (1 point) The A and B alleles in ABO blood types can give rise to an individual that is blog type AB. This specific blood type is an example of: A) multiple alleles B) epistasis C) codominance D) partial dominance Imagine the gene encoding the lac repressor was mutated so that lactose (more technically allolactose) no longer bound to the repressor. However, the lac repressor was still capable of binding DNA at the operator sequence....
2.) Activator and helper. Figure 19.29B gives the fold change for a genetic network with an...
2.) Activator and helper. Figure 19.29B gives the fold change
for a genetic network with an activator and helper. However, it
contains several mistakes that we will fix here.
a.) Derive the formula for the fold change under the assumptions
of a weak promoter and �!"# $% ≫ �. Show that the fold change is
given by the formula below rather than that in the book. Hint: The
schematic in Figure 19.29B should be helpful in determining the
states and...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
Consider what you know about the lac operon, and predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels of transcription. and the activator is b. When lactose and glucose are...
Consider an E. coli strain carrying a mutation in crp (encoding the catabolite activator protein) that prevents CAP from binding CAMP. Predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation test. The first F' plasmid contains the entire lac operon from a strain that is lacz, while the second F' plasmid contains the entire lac operon from a strain that is lacY. You test the ability of the transformed bacteria to grow on media where the sole carbon source is either glucose or lactose, and obtain the following results: Lac t mutant compl lacZ...
alllll them please all
To MOST readily demonstrate transformation of bacteria in the laboratory one could extract DNA from: A) his cells and add the DNA to his cells, then grow the cells on plates with histidine B) hist cells and add the DNA to his cells, then grow the cells on plates without histidine C) lac" cells and add the DNA to lact cells, then grow the cells on plates without histidine D) amp cells and add the DNA...
all them please
Question 23 (1 point) The A and B alleles in ABO blood types can give rise to an individual that is blog type AB. This specific blood type is an example of: A) multiple alleles B) epistasis C) codominance D) partial dominance Imagine the gene encoding the lac repressor was mutated so that lactose (more technically allolactose) no longer bound to the repressor. However, the lac repressor was still capable of binding DNA at the operator sequence....
2.) Activator and helper. Figure 19.29B gives the fold change
for a genetic network with an activator and helper. However, it
contains several mistakes that we will fix here.
a.) Derive the formula for the fold change under the assumptions
of a weak promoter and �!"# $% ≫ �. Show that the fold change is
given by the formula below rather than that in the book. Hint: The
schematic in Figure 19.29B should be helpful in determining the
states and...
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