Summary statement for the biological principles from the Enzyme Activity Lab.
Enzymes are biocatalyst which increases the rate of biochemical reactions. Hence enzymes are very important in organisms. Main principle of increasing rate of reaction is that every substrate(reactant in chemistry) are started to give products on guving certain amount of energy called Activation energy. Enzymes makes complex with substrate and lower the activation energy to start the reaction at lower energy investment and increases the rate of reaction.
Other factors which affact the activity of enzymes are -
Temprature - The activity of enzyme generally increases with increase in temperature but at very high tempratures enzymes are denatured to decrease enzyme activity.
pH also affacts the activity of enzyme.
Enzymes are very specific about their substrate and acts only on specific substrate molecules.
Summary statement for the biological principles from the Enzyme Activity Lab.
A summary statement about the conditions necessary for the action of enzyme
COST-LAB QUESTIONS: 1. What happens to enzyme activity as the enzyme concentration is increased? 2. Based on your results, what is the optimum pH for lactase? 3. Do all enzymes in the body have the same optimum pH? Why or why not? 4. What happens to enzyme activity as the substrate concentration is increased? Explain why this occurs.
In the enzyme activity of lactase lab, sodium carbonate was used to halt the enzyme-catalyzed reaction. Which of the following is the most likely explanation for sodium carbonate having this effect? A. Sodium carbonate raises the kinetic energy of the solution B. Sodium carbonate is a base and enzymes have an optimal pH range C. Sodium carbonate is toxic and quickly destroys the enzyme D. Sodium carbonate is able to lower the activation energy of the reaction
Mitochondrial enzyme- cytochrome c oxidase activity lab 1. What are the four main components of complex 4 or cytochrome c oxidase? 2. What happened to the absorption 55nm after addition of mitochondrial preparation and why?
When running enzyme assays, why do you need to normalize enzyme activity to total protein concentration? What does the specific activity mean in the research lab where enzymes are used? (Hint: are enzymes always pure?)
red suradienolides are described as cardiacglycosides owing to the similarity in their biological activity, the increase in the contractile force of the heart by inhibiting the enzyme Na+, K+-ATPase. Assign chirality (Ror S) to the asymmetric carbons present in the circled areas of the structure of Bufadienolide below? (choose the correct listed set of chirality in order from A, B, C, and D labels on the structure) 2.00 tion Select one: a S, R, RS b. S.RRR C. R, S,...
explain the induced-fit model of enzyme action, and how this
differe from the "Lock-&-key" model
NOA HOJAVEN EN GENOSSO Questions to expand your mind. 1. Explain the Induced-Fit model of enzyme action, and how this differs from the Lock-&-Key" model. 2. Distinguish between catabolic and anabolie enzyme reactions 3. Define and describe 3 variables that may affect enzyme activity. 4. **Online Search Opportunity** What are the steps involved in commercial cheese making? Is this different from how organic farm cheese...
why is DNS used in enzyme activity and why is it important for determining enzyme activity.
Bufadienolides are described as cardiacglycosides owing to the similarity in their biological activity, the increase in the contractile force of the heart by inhibiting the enzyme Na+, K+-ATPase. Assign chirality (Ror S) to the asymmetric carbons present in the circled areas of the structure of Bufadienolide below? (choose the correct listed set of chirality in order from A, B, C, and D labels on the structure) II Select one: a. R, S, S, S b. R, S, R, S C....
Microbiology In the competitive inhibition of enzyme activity, which statement is correct? Inhibitor directly competes with the substrate. Less substrates must be added in order to reach Vmax. The number of active sites is unlimited. None of the above. In the noncompetitive inhibition of enzyme activity, which statement is correct?Inhibitors will cause a conformational change in the enzyme, more substrate must be added to reach Vmax, Enzymes bound to the inhibitor can still bind substrate, none of the above. ....