Homework Answers
Ans 2. Specific single. Stranded DNA primer
Use PCR to produce single stranded DNA (using a single primer with a double stranded template).
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Which of the following components is NOT used in the technique of PCR to amplify a...
Which of the following is needed for the PCR technique to work? (multiple answers) a. A...
Which of the following is needed for the PCR technique to work? (multiple answers) a. A probe that can anneal to the center of the target DNA sequence. b. A thermostable RNA polymerase c. Large amounts of pure DNA d. A thermostable DNA polymerase e. Two DNA primers that can anneal to the target DNA on either side of the DNA to be amplified. f. A thermostable DNA ligase
EXPLAIN each answer thoroughly. There are two common goals when using PCR to amplify DNA. A....
EXPLAIN each answer thoroughly. There are two common goals when using PCR to amplify DNA. A. Make lots of copies of a specific DNA sequence to use in cloning (preparative PCR) B. Detect the presence or relative amount of a specific gene under varying conditions (analytical PCR) *Remember: PCR is very similiar to DNA replication, but uses a DNA polymerase to amplify only specific parts of DNA sequence based on the sequence of the primers. 3. For which goal (A...
Now. you should be able to answer the following questions: • How the amplification will be...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
1.The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the...
1.The PCR (polymerase chain reaction) protocol that is currently
used in laboratories was facilitated by the discovery of a
bacterium called Thermus aquaticus in a hot spring inside
Yellowstone National Park, in Wyoming. This organism contains a
heat-stable form of DNA polymerase known as Taq
polymerase, which continues to function even after it has been
heated to 95°C.
a.Why would such a heat-stable polymerase be beneficial in
PCR?
b.What would happen if it weren’t
heat stable?
c.How might you choose...
. Review your notes and the introduction for this lab regarding the components of PCR reactions....
. Review your notes and the introduction for this lab regarding the components of PCR reactions. a. What is the function of primers in a PCR reaction? PCR primers is to provide a "free" 3'-OH group to which the DNA polymerase can add dNTPs b. What is the function of DNA polymerase in a PCR reaction? What specific DNA polymerase is used in PCR and why? c. What are nucleotides and what is the function of nucleotides in a PCR...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)?...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)? Question 16 options: A) Nucleoside triphosphates that serve as the source of the nucleotides A, T, C, and G needed in the synthesis of the new strands of DNA B) A restriction endonuclease enzyme that cleaves DNA at specific locations C) The segment of DNA that must be copied D) A DNA polymerase enzyme that will catalyze the synthesis of a complementary strand of...
Pick a pair of primers that you could use to amplify the following double-stranded DNA sequence...
Pick a pair of primers that you could use to amplify the
following double-stranded DNA sequence using PCR. Note: Both
strands shown below.
5'-CCACTAGGGAGCTACGTAGGCACGGCATTACACGGATAGGCATTAACG-3'
3'-GGTGATCCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGTAATTGC-5'
Pick a pair of primers that you could use to amplify the following double-stranded DNA sequence using PCR. Note: Both strands shown below 5'-CCACTAGGGAGCTACGTAGGCACGGCATTACACGGATAGGCATTAACG-3 3'-GGTGATCCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGTAATTGC-5 5'-CCACTAG-3'; 5'-CGTTAAT-3' 5'-GGTGATC-3'; 5'-GCAATTA-3' 5'-GATCACC-3':5'-CGTTAAT-3' 5'-CCACTAG-3';5'-GCAATTA-3' 5'-GGTGATC-3';5'-CGTTAAT-3'
Select the components and equipment that would be necessary for doing PCR. select all that apply...
Select the components and equipment that would be necessary for doing PCR. select all that apply Sample containing the DNA to be amplified dNTPs (deoxyribonucleotide triphosphates) Taq polymerase (thermostable polymerase) RNA polymerase Restriction endonucleases Primers specific to the DNA sequence to be amplified Thermocycler
1. Explain why, when PCR is used to amplify the same region of DNA from two...
1. Explain why, when PCR is used to amplify the same region of DNA from two different people, the size of the DNA fragment(s) generated may be different? 2. What characteristic of the DNA molecule makes it possible to use electrophoresis to separate DNA molecules by size? Explain why this characteristic is important for electrophoresis and what part of the DNA molecule creates this characteristic. 3. You are performing PCR. After four cycles of PCR, how many double-stranded copies of...
10. For each of the following reagents briefly explain their role in a PCR reaction (3...
10. For each of the following reagents briefly explain their role in a PCR reaction (3 points each) a. dNTP b. DNA containing the sequence to be amplified. c. DNA ligase. d. Heat-stable DNA polymerase. e. Oligonucleotide primer(s).
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
1.The PCR (polymerase chain reaction) protocol that is currently
used in laboratories was facilitated by the discovery of a
bacterium called Thermus aquaticus in a hot spring inside
Yellowstone National Park, in Wyoming. This organism contains a
heat-stable form of DNA polymerase known as Taq
polymerase, which continues to function even after it has been
heated to 95°C.
a.Why would such a heat-stable polymerase be beneficial in
PCR?
b.What would happen if it weren’t
heat stable?
c.How might you choose...
. Review your notes and the introduction for this lab regarding the components of PCR reactions. a. What is the function of primers in a PCR reaction? PCR primers is to provide a "free" 3'-OH group to which the DNA polymerase can add dNTPs b. What is the function of DNA polymerase in a PCR reaction? What specific DNA polymerase is used in PCR and why? c. What are nucleotides and what is the function of nucleotides in a PCR...
Pick a pair of primers that you could use to amplify the
following double-stranded DNA sequence using PCR. Note: Both
strands shown below.
5'-CCACTAGGGAGCTACGTAGGCACGGCATTACACGGATAGGCATTAACG-3'
3'-GGTGATCCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGTAATTGC-5'
Pick a pair of primers that you could use to amplify the following double-stranded DNA sequence using PCR. Note: Both strands shown below 5'-CCACTAGGGAGCTACGTAGGCACGGCATTACACGGATAGGCATTAACG-3 3'-GGTGATCCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGTAATTGC-5 5'-CCACTAG-3'; 5'-CGTTAAT-3' 5'-GGTGATC-3'; 5'-GCAATTA-3' 5'-GATCACC-3':5'-CGTTAAT-3' 5'-CCACTAG-3';5'-GCAATTA-3' 5'-GGTGATC-3';5'-CGTTAAT-3'
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