Which of the following is needed for the PCR technique to work? (multiple answers) a. A...

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Which of the following is needed for the PCR technique to work? (multiple answers) a. A...

Which of the following is needed for the PCR technique to work? (multiple answers)

	a.	A probe that can anneal to the center of the target DNA sequence.
	b.	A thermostable RNA polymerase
	c.	Large amounts of pure DNA
	d.	A thermostable DNA polymerase
	e.	Two DNA primers that can anneal to the target DNA on either side of the DNA to be amplified.
	f.	A thermostable DNA ligase

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Answer 1

Answer #1

PCR is a very sensitive technique of DNA amplification so it needs a very little amount of DNA, In addition it needs two DNA primers, thermostable DNA and free nucleotides called ddNTPs. So here the correct options are (d) and (e).

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Answer 2

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