Which of the following statements about the polymerase chain reaction (PCR) is false? a The DNA...
Which of the following statements about the polymerase chain reaction (PCR) is false?
| a | The DNA ligase used is from a thermophilic bacteria so that it can resist denaturation at high temperatures. |
| b | Newly synthesized DNA must be heat-denatured before the next round of DNA synthesis begins. |
| c | The boundaries of the amplified DNA segment are determined by the synthetic oligonucleotides used to prime DNA synthesis. |
| d | DNA amplified by PCR can be cloned. |
Homework Answers
Answer:- In case of polymerase chain reaction taq-polymerase is an enzyme which is isolated from the bacteria thermus aquaticus rather than the DNA ligase.
Hence option A is false statement with respect to PCR.
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heated to 95°C.
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