Why is a heat-stable DNA polymerase from a thermophilic bacterium (the Taq polymerase) used in the...
Why is a heat-stable DNA polymerase from a thermophilic bacterium (the Taq polymerase)
used in the PCR rather than a DNA polymerase from E. coli or humans? PCR involves heating
the reaction at the beginning of each cycle to separate the newly synthesized ds DNA into
single strands so that they can act as templates for the next round.
a. UsingTaq avoids having to add it afresh for each round of DNA replication.
b.Taq allows a faster transcription
c. Taq is specific for RT-PCR
d. none of them
Homework Answers
Ans option D . None of these
Actually Taq polymerase is isolated from a heat loving thermophillic bacteria known as Thermus aquaticus loving in natural hot spring.This enzyme is thermostable and this enzyme does not break down at high temperature required in pcr.
Add Answer to:
Why is a heat-stable DNA
polymerase from a thermophilic bacterium (the Taq
polymerase)
used in the...
1. (a) Assume that during DNA replication in a bacterium a mistake is made and a...
1. (a) Assume that during DNA replication in a bacterium a mistake is made and a G is inserted into the newly synthesized DNA strand opposite a T in the template DNA strand. If this mistake is not repaired before the next round of DNA replication, what mutation will eventually result? Show your work (b) What type of mutation is the resulting mutation? (transversion, transition, indel, base substitution) 2. (a) What is the purpose of Taq polymerase in a PCR...
1.The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the...
1.The PCR (polymerase chain reaction) protocol that is currently
used in laboratories was facilitated by the discovery of a
bacterium called Thermus aquaticus in a hot spring inside
Yellowstone National Park, in Wyoming. This organism contains a
heat-stable form of DNA polymerase known as Taq
polymerase, which continues to function even after it has been
heated to 95°C.
a.Why would such a heat-stable polymerase be beneficial in
PCR?
b.What would happen if it weren’t
heat stable?
c.How might you choose...
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded...
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 21. Which enzyme joins newly synthesized DNA fragments on the lagging strand? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 22. In a PCR reaction, at which temperature do the two strands of DNA...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
1. (a) Assume that during DNA replication in a bacterium a mistake is made and a G is inserted into the newly synthesized DNA strand opposite a T in the template DNA strand. If this mistake is not repaired before the next round of DNA replication, what mutation will eventually result? Show your work (b) What type of mutation is the resulting mutation? (transversion, transition, indel, base substitution) 2. (a) What is the purpose of Taq polymerase in a PCR...
1.The PCR (polymerase chain reaction) protocol that is currently
used in laboratories was facilitated by the discovery of a
bacterium called Thermus aquaticus in a hot spring inside
Yellowstone National Park, in Wyoming. This organism contains a
heat-stable form of DNA polymerase known as Taq
polymerase, which continues to function even after it has been
heated to 95°C.
a.Why would such a heat-stable polymerase be beneficial in
PCR?
b.What would happen if it weren’t
heat stable?
c.How might you choose...
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 21. Which enzyme joins newly synthesized DNA fragments on the lagging strand? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 22. In a PCR reaction, at which temperature do the two strands of DNA...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
Most questions answered within 3 hours.
-
Consider the reaction, C3 H8 + O2 --> CO2 + H2O. How many
moles of O2...
asked 21 minutes ago -
You and your opponent both roll a fair die. If you both roll the
same number,...
asked 38 minutes ago -
In a study of the accuracy of fast food drive-through orders,
Restaurant A had 257 accurate...
asked 38 minutes ago -
Identify and describe in detail the four categories of
institutions that could be included in a...
asked 44 minutes ago -
In python
class Customer:
def __init__(self, customer_id, last_name, first_name, phone_number, address):
self._customer_id = int(customer_id)
self._last_name =...
asked 51 minutes ago -
What is an example of a limitation in implementing a new
ERP system and how it...
asked 46 minutes ago -
In a section of 9.7cm of an artery with a radius of 2.6mm there
is a...
asked 47 minutes ago -
the two carboxylic acid groups of aspartic acid have different
acidities with pKa values of 2.1...
asked 51 minutes ago -
Would CuCO3 aqueous salt combined with calcium chloride
form a solid precipitate? If so, what would...
asked 50 minutes ago -
How do ECM Solutions assist in embedding a culture of continuous
improvement in an organization? (Project...
asked 1 hour ago -
Directions
These directions introduce the idea of Essential Questions.
Since this may be a new concept...
asked 1 hour ago -
1.b. Fiscal policy is said to suffer from ‘crowding out’.
Explain what this means and why...
asked 1 hour ago