This week you will be preparing 500mL of electrode tank buffer containing 25mM Tris, 190mM glycine...
This week you will be preparing 500mL of electrode tank buffer containing 25mM Tris, 190mM glycine and 0.1% SDS. The amount of 10% SDS stock solution that needs to be added is
10mL
0.5mL
5mL
1mL
Homework Answers
We have to use the serial dilution method
C1 V1 = C2 V2
Here we have to prepare 0.1% SDS solution from 10%SDS stock solution for making 500 ml buffer.
Therefore we have now C1= 10 ,V1 ? , C2 = 0.1and V2 = 500
C1 V1 = C2V2
10× V1 =.0.1 ×500
V1 = (0.1×500)÷10
V1= 5 ml
Therefore the amount of 10% SDS stock solution that needs to be added is 5 ml
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