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You have discovered a new mutant strain of E.colithat has a mutation in the trpL gene,...

  1. You have discovered a new mutant strain of E.colithat has a mutation in the trpL gene, so that there are no trp codons in the resulting mRNA transcribed from this gene. Explain how this might affect the attenuation / regulation of the trp gene expression.
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The trpL sequence is essential for terminating and anti-terminating the trp operon if the tryptophan concentration is slightly efficient and that can be adequate by the trpL coded tryptophan. The attenuator sequence work only when the trpL sequence is transcribed. Therefore, if trpL sequence is not working, then the attenuator sequence will also not work. In the absence of tryptophan, the trp operon will work.

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