2) How is the Ames test useful in investigating the mutagenicity of common substances?
2) How is the Ames test useful in investigating the mutagenicity of common substances?
Homework Answers
The Ames test is a widely employed method that uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism. More formally, it is a biological assay to assess the mutagenicpotential of chemical compounds.[1] A positive test indicates that the chemical is mutagenic and therefore may act as a carcinogen, because cancer is often linked to mutation. The test serves as a quick and convenient assay to estimate the carcinogenic potential of a compound because standard carcinogen assays on mice and rats are time-consuming (taking two to three years to complete) and expensive. However, false-positives and false-negatives are known.
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2) How is the Ames test useful in investigating the mutagenicity
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Can anyone help me to finish my Ames Test Lab Assignment NO.1/2/3. All data and information...
Can anyone help me to finish my Ames Test Lab Assignment
NO.1/2/3. All data and information are posted below! Thanks!
/20 marks Ames Test Assignment Name: Data Tables Table 1: Number of colonies counted on negative control plates. (2 marks) Number of Colonies from your plate & class Negative Control Chemical Zi 0 dH20 0 Average Number of Colonies67 Table 2: Number of colonies counted, number of induced mutations, and degree of mutagenicity of chemicals on test plates. (6 marks)...
Below are results of the Ames Test that was conducted to determine whether a compound called...
Below
are results of the Ames Test that was conducted to determine
whether a compound called sodium azide could be useful for causing
detrimental mutations in bacteria, thus preventing bacterial growth
on surgical tools. To test the mutagenicity of sodium azide, the
Ames test was carried out. The bacterial species Salmonella
typhimurium requires Histidine to grow and was plated on minimum
growth plates lacking histidine. Bacteria plated on the control
plate had only buffer added, while bacteria on the treatment...
Why is it important to investigate the mutagenicity of common substances?
Why is it important to investigate the mutagenicity of common substances?
1. You are screening three new food additives for potential mutagenicity using the Ames test. You...
1. You are screening three new food additives for potential mutagenicity using the Ames test. You used a his-s. typhimurium bacterium for this test and obtained the following results: Additive + Control (HO) Additive Rat liver extract Additive 1 Additive 2 311 Additive 3 250 260 What would vour conclusions be concerning each of the additives given the data provided above? 22 24
What is the purpose of the Ames test? How are his- bacteria used in this test?
What is the purpose of the Ames test? How are his- bacteria used in this test?
core: 42.5% Resources Hint Check Question 12 of 14 > The Ames test determines the frequency...
core: 42.5% Resources Hint Check Question 12 of 14 > The Ames test determines the frequency with which a chemical causes tutions in DNA. The results of the Ames test for the substances A, B, and C are provided. Label the carcinogenic potential of each of these substances based on the production of his revertants in the presence or absence of liver extract (LE). his strain + substance A - LE + LE his strain + substance B - LE...
Below is a modified version of the Ames test where instead of soaking a filter paper...
Below is a modified version of the Ames test where instead of
soaking a filter paper with the test compound, the test compound is
layered over the top of the plate. The top left plate was layered
with liver extract only. The top right plate was not layered with
anything. The bottom right plate was layered with the untreated
test compound and the bottom left plate was layered with the test
compound after treating it with liver extract.
What can...
After performing an Ames test on 2 unknown test chemicals, suggest a follow-up experiment that can...
After performing an Ames test on 2 unknown test chemicals, suggest a follow-up experiment that can uncover more detail. (can be a variation of the test or something new)
1) The talented student Lin has conducted the Ames test for two components: GT-1 and GT-2....
1) The talented student Lin has conducted the Ames test for two components: GT-1 and GT-2. The results are shown in the table below. GT-1 GT-2 Control (native media) Explain the experiment results and determine if the compounds are suspected as mutagens. In your answer, describe the Ames test procedure and rational.
1.What are some advantages and disadvantages of the Ames test over tests for carcinogens that use...
1.What are some advantages and disadvantages of the Ames test over tests for carcinogens that use animal models? 2.Define the following terms: a.Mutagen b.Carcinogen c.Auxotroph d.Prototroph e.Back-mutation
Can anyone help me to finish my Ames Test Lab Assignment
NO.1/2/3. All data and information are posted below! Thanks!
/20 marks Ames Test Assignment Name: Data Tables Table 1: Number of colonies counted on negative control plates. (2 marks) Number of Colonies from your plate & class Negative Control Chemical Zi 0 dH20 0 Average Number of Colonies67 Table 2: Number of colonies counted, number of induced mutations, and degree of mutagenicity of chemicals on test plates. (6 marks)...
Below
are results of the Ames Test that was conducted to determine
whether a compound called sodium azide could be useful for causing
detrimental mutations in bacteria, thus preventing bacterial growth
on surgical tools. To test the mutagenicity of sodium azide, the
Ames test was carried out. The bacterial species Salmonella
typhimurium requires Histidine to grow and was plated on minimum
growth plates lacking histidine. Bacteria plated on the control
plate had only buffer added, while bacteria on the treatment...
1. You are screening three new food additives for potential mutagenicity using the Ames test. You used a his-s. typhimurium bacterium for this test and obtained the following results: Additive + Control (HO) Additive Rat liver extract Additive 1 Additive 2 311 Additive 3 250 260 What would vour conclusions be concerning each of the additives given the data provided above? 22 24
core: 42.5% Resources Hint Check Question 12 of 14 > The Ames test determines the frequency with which a chemical causes tutions in DNA. The results of the Ames test for the substances A, B, and C are provided. Label the carcinogenic potential of each of these substances based on the production of his revertants in the presence or absence of liver extract (LE). his strain + substance A - LE + LE his strain + substance B - LE...
Below is a modified version of the Ames test where instead of
soaking a filter paper with the test compound, the test compound is
layered over the top of the plate. The top left plate was layered
with liver extract only. The top right plate was not layered with
anything. The bottom right plate was layered with the untreated
test compound and the bottom left plate was layered with the test
compound after treating it with liver extract.
What can...
1) The talented student Lin has conducted the Ames test for two components: GT-1 and GT-2. The results are shown in the table below. GT-1 GT-2 Control (native media) Explain the experiment results and determine if the compounds are suspected as mutagens. In your answer, describe the Ames test procedure and rational.
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