What is the purpose of the Ames test? How are his- bacteria used in this test?
What is the purpose of the Ames test? How are his- bacteria used in this test?
Homework Answers
Ames test is a method of assaying the mutagenic ability of
chemical compounds. This test is based on the ability of reverse
mutation or back mutation.
In Ames test a strain of bacteria is used particularly
Salmonella typhimurium having mutation in gene responsible
for histidine synthesis. These auxotrophs are his- and require the
presence of histidine in the growth medium. His- strain is cultured
in the presence of chemical to be assayed to observe the effect of
the chemical on the bacterial ability to resynthesise the
histidine. If the chemical is able to induce reverse mutation in
the bacteria so that it can synthesise histidine then this chemical
is a potential mutagen.
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What is the purpose of the Ames test? How are his- bacteria used
in this test?
What is the purpose of the AMES test ? Why is liver homogenate used ? If...
What is the purpose of the AMES test ? Why is liver homogenate used ? If you had an opportunity to conduct the test , is there a particular substance that you use or come in contact with daily that you would test? If the substance tested had a positive result would you stop using?
In an Ames test using his- Salmonella bacteria a researcher determines that adding a test compound...
In an Ames test using his- Salmonella bacteria a researcher determines that adding a test compound plus the S9 extract produces a large number of his+ revertants, but mixing the his- strain plus the test compound without adding S9 does not produce an elevated number of his+ revertants. Explain this result. Also state whether or not you think this compound should be considered a potential mutagen.
What is the Ames test and why is it commonly used by the FDA?
What is the Ames test and why is it commonly used by the FDA?
The researchers grow and maintain the Ames test bacteria in liquid media containing histidine. They then...
The researchers grow and maintain the Ames test bacteria in liquid media containing histidine. They then prepare plates (semi-solid media) with media that lacks histidine. They also prepare tubes of media that is between the semi-solid and liquid media (soft agar) that contains trace amounts of histidine. They inoculate tubes of the soft agar with the mutant Ames test bacteria and add one of the following - 100 l of (1) chemical A in a solvent, (2) chemical B in...
1.What is the difference between a transition and a transversion? Which type of base substitution is...
1.What is the difference between a transition and a transversion? Which type of base substitution is more common? 2.What is the difference between a missense mutation and a nonsense mutation? Between a silent mutation and a neutral mutation? 3.What is the purpose of the Ames test? How are his-bacteria used in this test?
Below are results of the Ames Test that was conducted to determine whether a compound called...
Below
are results of the Ames Test that was conducted to determine
whether a compound called sodium azide could be useful for causing
detrimental mutations in bacteria, thus preventing bacterial growth
on surgical tools. To test the mutagenicity of sodium azide, the
Ames test was carried out. The bacterial species Salmonella
typhimurium requires Histidine to grow and was plated on minimum
growth plates lacking histidine. Bacteria plated on the control
plate had only buffer added, while bacteria on the treatment...
Mutations are heritable changes in DNA. They are essential to the study of genetics and are...
Mutations are heritable changes in DNA. They are essential to the study of genetics and are useful in many other biological fields. How are mutations used to help in understanding basic biological processes? What is the SOS system and how does it lead to an increase in mutations? What is the purpose of the Ames test? How are his- bacteria used in this test?
You just found a new compound “ABC” to be carcinogenic via the Ames test using His-Salmonella....
You just found a new compound “ABC” to be carcinogenic via the Ames test using His-Salmonella. Which of the experimental plates listed below will have the most colonies? His-Salmonella, rat liver enzymes His-Salmonella, rat liver enzymes, compound “ABC” His-Salmonella, compound “ABC” What test can you do to confirm that “ABC is carcinogenic?
2) How is the Ames test useful in investigating the mutagenicity of common substances?
2) How is the Ames test useful in investigating the mutagenicity of common substances?
How is this test used diagnostically? Which bacteria is more virulent and why? using the Catalase...
How is this test used diagnostically? Which bacteria is more virulent and why? using the Catalase test and the bacterias being Staphylococcus aureus and Staphylcocccus epidermidis
Below
are results of the Ames Test that was conducted to determine
whether a compound called sodium azide could be useful for causing
detrimental mutations in bacteria, thus preventing bacterial growth
on surgical tools. To test the mutagenicity of sodium azide, the
Ames test was carried out. The bacterial species Salmonella
typhimurium requires Histidine to grow and was plated on minimum
growth plates lacking histidine. Bacteria plated on the control
plate had only buffer added, while bacteria on the treatment...
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