Question

Can anyone help me to finish my Ames Test Lab Assignment NO.1/2/3. All data and information are posted below! Thanks!

/20 marks Ames Test Assignment Name: Data Tables Table 1: Number of colonies counted on negative control plates. (2 marks) Number of Colonies from your plate & class Negative Control Chemical Zi 0 dH20 0 Average Number of Colonies67 Table 2: Number of colonies counted, number of induced mutations, and degree of mutagenicity of chemicals on test plates. (6 marks) Test Chemical (not including dH20) Number of Number of Induced Colonies Relative Mutagenicity Mutations 2 rHeine hilo hne a ala zhenolghthulean

Answer 1

1. The strain of Salmonella that you are using is defective in cell wall synthesis and DNA damage repair. Why are these useful characteristics for this experiment? (2) Ames test is used to determine the carcinogenic or mutagenic chemicals. The strain of Salmonella used in Ames test is defective in cell wall synthesis and DNA damage repair because these strains are more vulnerable to the exogenous mutagens and cannot reverse the mutations caused by them due to defective mutation repair mechanism.