Question

35. To determine whether transcription of a certain gene varies in response to an environmental stimulus, the best option is to: A. Isolate DNA from the organism in the presence and absence of the stimulus and amplify it by PCR B. Perform a Southern blot on DNA isolated in the presence and absence of the C. Perform transposon mutagenesis D. Fuse the promoter of the gene to a reporter construct and check reporter activity in E. Compare the gene sequences in DNA isolated in the presence and absence of the stimulus. the presence and absence of the stimulus stimulus.

Answer 1

D is best option as reporter gene will encode a phenotype from which easily we can know transcription durund stimulus